Xin K Q, Morikawa S, Fang Z Y, Mukoyama A, Okuda K, Ushijima H
National Institute of Health, Tokyo, Japan.
Virology. 1993 Dec;197(2):813-6. doi: 10.1006/viro.1993.1663.
Sequence analysis of the gene encoding the major neutralization glycoprotein (VP7) was performed on 12 human isolates of serotype 1 of rotavirus in Japan and China. They were examined for genetic variations among serotype 1 isolates. Comparative studies of their nucleotide and deduced amino acid sequences between the 12 isolates and the Wa strain revealed an overall homology of more than 92 and 96%, respectively. Higher degrees of homologies were observed between Wa and 2 strains (K1 and K2) in Tokyo, 1979-1980, than between Wa and recent isolated strains in Tokyo and in China. In our isolates, a total of 16 amino acid residues frequently converted to another amino acid. Six amino acid residues belonging to the major neutralizing epitope regions (B, D, and E in this communication) frequently converted. From these data three subtypes (subtypes A, B, and intermediate) were suggested to be divided. Whether these differences are an important mechanism in the epidemiology of rotaviruses requires further investigation.
对日本和中国的12株1型轮状病毒的人分离株进行了编码主要中和糖蛋白（VP7）基因的序列分析。检测了1型分离株之间的基因变异。对这12株分离株与Wa株之间的核苷酸序列和推导的氨基酸序列进行比较研究，结果显示总体同源性分别超过92%和96%。1979 - 1980年东京的Wa株与2株（K1和K2）之间的同源性高于Wa株与东京和中国近期分离株之间的同源性。在我们的分离株中，共有16个氨基酸残基经常转变为另一种氨基酸。属于主要中和表位区域（本通讯中的B、D和E）的6个氨基酸残基经常转变。根据这些数据，建议分为三个亚型（A亚型、B亚型和中间亚型）。这些差异是否是轮状病毒流行病学中的一个重要机制，需要进一步研究。
