Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
J Med Virol. 2010 May;82(5):878-85. doi: 10.1002/jmv.21630.
Sequence and phylogenetic analyses of the rotavirus VP7 gene were performed on 52 human G2 and G4 strains isolated in Japan, China, Thailand, and Vietnam during 2001-2003. All genotype G2 strains included in the study clustered into lineage II of the phylogenetic tree, together with the majority of global G2 strains detected since 1995. The amino acid substitution at position 96 from aspartic acid to asparagine was noted among the emerging or re-emerging G2 rotavirus strains in Japan, Thailand, and Vietnam during 2002-2003. Genotype G4 strains detected in Vietnam grouped into lineage Ia of the phylogenetic tree, whereas Japanese G4 strains clustered in lineage Ic which included emerging G4 strains from Argentina, Italy, Paraguay, and Uruguay. It is noteworthy that an insertion of asparagine was found at position 76 in all the Japanese strains and that its presence might be involved in the emergence of G4 rotavirus in Japan during 2002-2003.
对 2001-2003 年期间在日本、中国、泰国和越南分离的 52 株人轮状病毒 G2 和 G4 株的 VP7 基因进行了序列和系统进化分析。本研究中包含的所有 G2 基因型毒株均聚类于系统进化树的 II 谱系，与 1995 年以来检测到的大多数全球 G2 毒株一致。2002-2003 年期间，在日本、泰国和越南新出现或再次出现的 G2 轮状病毒株中观察到第 96 位天冬氨酸突变为天冬酰胺。在越南检测到的 G4 基因型毒株聚类于系统进化树的 Ia 谱系，而日本的 G4 毒株聚类于 Ic 谱系，该谱系包括来自阿根廷、意大利、巴拉圭和乌拉圭的新出现的 G4 株。值得注意的是，所有日本株均在第 76 位发现天冬酰胺插入，其存在可能与 2002-2003 年日本 G4 轮状病毒的出现有关。
