Suo Mao-rong, Tian Ze, Yang Jun-shan, Lu Yang, Wu Lü, Li Wu
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China.
Yao Xue Xue Bao. 2007 Feb;42(2):166-70.
To search for bioactive compounds from the flower disc of Helianthus annuus L., chromatography was used to isolate and purify the chemical constituents, their structures were identified by spectral analysis, MTT method was applied to investigate their cytotoxic activities, some compounds showed moderate cytotoxic activities on SF-268, MCF-7 and HepG2 cell lines. Eleven compounds were obtained from the flower disc of H. annuus, and identified as ent-kaurane-2alpha, 16alpha-diol (1) and entkaurane-15alpha,16alpha-epoxy-17-al-19-oic acid (2), and nine known diterpenes, ent-kaurane-16P-ol (3), phyllocladan-16beta-ol (4) , ent-atisan-16a-ol (5) , grandifloric acid (6) , angeloylgrandifloric acid (7), ent-kaurane-16-en-19-oic acid (8), ent-kaurane-17-hydroxy-15-en-19-oic acid (9), ent-kaurane-16beta, 17-dihydroxy-19-oic acid (10), and ciliaric acid (11). Compounds 1 and 2 are new compounds, some compounds showed cytotoxic activities on SF-268, MCF-7 and HepG2 cell lines.
为了从向日葵花盘中寻找生物活性化合物，采用色谱法分离纯化化学成分，通过光谱分析鉴定其结构，应用MTT法研究其细胞毒性活性，部分化合物对SF-268、MCF-7和HepG2细胞系表现出中等细胞毒性活性。从向日葵花盘中获得了11种化合物，分别鉴定为对映贝壳杉烷-2α,16α-二醇（1）和对映贝壳杉烷-15α,16α-环氧-17-醛-19-酸（2），以及9种已知的二萜类化合物，对映贝壳杉烷-16β-醇（3）, 扁枝杉烷-16β-醇（4）, 对映阿替生-16α-醇（5）, 大花酸（6）, 当归酰大花酸（7）, 对映贝壳杉烷-16-烯-19-酸（8）, 对映贝壳杉烷-17-羟基-15-烯-19-酸（9）, 对映贝壳杉烷-16β,17-二羟基-19-酸（10）, 以及睫状酸（11）。化合物1和2为新化合物，部分化合物对SF-268、MCF-7和HepG2细胞系表现出细胞毒性活性。
