Pal Kunal, Banthia A K, Majumdar D K
Materials Science Centre, Indian Institute of Technology, Kharagpur, India.
J Mater Sci Mater Med. 2007 Sep;18(9):1889-94. doi: 10.1007/s10856-007-3061-2. Epub 2007 May 24.
The wound is a biosynthetic environment in which numerous cellular processes are interlinked in the process of repair. Modern dressings are designed to facilitate wound healing rather than just to cover it. Hydrogel dressing can protect injured skin and keep it appropriately moist to speed the healing process by absorbing exudates while maintaining the products of tissue repair, including growth factor and lysosomes, in contact with the wound. The design and development of novel membrane of hydrogels prepared by esterification of polyvinyl alcohol with gelatin were attempted. Contact angle of goat blood was determined. The hydrogel was characterized by hemolysis test and water vapor transmission rate. Diffusion coefficient of salicylic acid (SA) and gatifloxacin, a fourth generation fluoroquinolone, through the membrane was determined. Both the drugs were used as model drug. Methyl tetrazolium dye assay of the membrane was done using L929 fibroblast cell line and mice splenocytes to establish the biocompatibility of the membrane. The equilibrium goat blood-in-air contact angles of measured ester films ranged from 56 to 60 degrees . The hydrogel was found to be hemocompatible and moisture retentive indicating its possible use in moist wound care. The diffusion coefficient of SA and gatifloxacin through the membrane was found to be 1.49 x 10(-5) and 3.97 x 10(-6) cm(2)/s respectively. The membrane was found to be compatible with the L929 fibroblast cell line and mice splenocytes.
伤口是一个生物合成环境，在修复过程中众多细胞过程相互关联。现代敷料旨在促进伤口愈合，而不仅仅是覆盖伤口。水凝胶敷料可以保护受损皮肤，并通过吸收渗出液使其保持适当的湿润，以加速愈合过程，同时使包括生长因子和溶酶体在内的组织修复产物与伤口保持接触。尝试通过聚乙烯醇与明胶的酯化反应制备新型水凝胶膜并进行设计和开发。测定了山羊血的接触角。通过溶血试验和水蒸气透过率对水凝胶进行了表征。测定了水杨酸（SA）和第四代氟喹诺酮类药物加替沙星通过该膜的扩散系数。两种药物均用作模型药物。使用L929成纤维细胞系和小鼠脾细胞对该膜进行甲基四氮唑染料试验，以确定该膜的生物相容性。所测酯膜的山羊血-空气平衡接触角范围为56至60度。发现该水凝胶具有血液相容性且能保持水分，表明其可能用于湿性伤口护理。发现SA和加替沙星通过该膜的扩散系数分别为1.49×10（-5）和3.97×10（-6）cm（2）/s。发现该膜与L929成纤维细胞系和小鼠脾细胞相容。
