Kankofer M, Radzki R P, Bieńko M, Albera E
Department of Animal Biochemistry and Physiology, Faculty of Veterinary Medicine, Agricultural University, 20-033 Lublin, Akademicka 13, Poland.
J Vet Med A Physiol Pathol Clin Med. 2007 Jun;54(5):225-9. doi: 10.1111/j.1439-0442.2007.00916.x.
The lack of protective action of oestrogens which appears during menopausal period may be the reason of serious metabolic disturbances including oxidative stress. The hypothesis was stated that ovariectomy may induce the variations of antioxidant/oxidant status which can be detected in rat liver. A total of 102 healthy Wistar female rats were included in the experiment and divided into control (CON; n = 6), sham-operated (SHO; n = 48) and ovariectomized (OVX; n = 48) groups. Animals from SHO (n = 6) and OVX (n = 6) groups were killed every week during 8 weeks of experiment to detect dynamic changes in examined parameters. Anti-oxidative enzyme activities [glutathione peroxidase (GSH-Px); superoxide dismutase (SOD)] as well as total antioxidant capacity (TAC) and the intensity of lipid peroxidation, measured by the concentration of N,N,diethyl-p-phenylene diamine (DEPPD) radical, were determined in liver homogenates by the use of spectrophotometric methods. Wave-like patterns of examined parameters within 8 weeks of experiment were detected. GSH-Px activity tended to be higher in OVX animals and was significantly lower at 8th week when compared with 1st week of experiment. SOD activity was higher in SHO animals and showed significant differences between 3rd, 4th, 7th and 1st week. TAC values were significantly higher in OVX when compared with SHO groups in 2nd, 4th, 5th week and significantly lower in 3rd, 6th and 7th week of the experiment. The concentration of DEPPD radicals tended to increase in OVX group. In conclusion, ovariectomy which leads to oestrogen insufficiency is reflected as well in variation of anti-oxidative/oxidative parameters in rat liver homogenates.
更年期出现的雌激素缺乏保护作用可能是包括氧化应激在内的严重代谢紊乱的原因。有人提出假说，卵巢切除术可能会引起抗氧化/氧化状态的变化，这种变化可以在大鼠肝脏中检测到。共有102只健康的Wistar雌性大鼠被纳入实验，并分为对照组（CON；n = 6）、假手术组（SHO；n = 48）和卵巢切除组（OVX；n = 48）。在实验的8周内，每周处死SHO组（n = 6）和OVX组（n = 6）的动物，以检测所检查参数的动态变化。通过分光光度法测定肝脏匀浆中的抗氧化酶活性[谷胱甘肽过氧化物酶（GSH-Px）；超氧化物歧化酶（SOD）]以及总抗氧化能力（TAC）和脂质过氧化强度（通过N，N，二乙基-对苯二胺（DEPPD）自由基浓度测定）。在实验的8周内检测到了所检查参数的波形模式。OVX动物的GSH-Px活性趋于较高，与实验第1周相比，在第8周时显著降低。SHO动物的SOD活性较高，在第3、4、7周和第1周之间存在显著差异。与SHO组相比，OVX组在实验的第2、4、5周TAC值显著更高，在第3、6、7周显著更低。OVX组中DEPPD自由基浓度趋于增加。总之，导致雌激素不足的卵巢切除术也反映在大鼠肝脏匀浆中抗氧化/氧化参数的变化上。
