Kara Ismail Oguz, Sahin Berksoy, Gunesacar Ramazan
Department of Medical Oncology, Cukurova University Faculty of Medicine, Adana, Turkey.
Adv Ther. 2007 Jan-Feb;24(1):29-40. doi: 10.1007/BF02849990.
Investigators in this study explored levels of soluble CD27 (sCD27), interleukin (IL)-8, and IL-10 in B-cell chronic lymphocytic leukemia (B-CLL), and the correlation of these levels with disease stage and prognosis. Plasma IL-8, IL-10, and sCD27 levels were assessed with enzyme-linked immunosorbent assay tests in 22 healthy donors and 70 patients with B-CLL (49 men and 21 women). Mean patient age was 61.57 y (range, 44-75 y). Mean healthy donor age was 62.09 y (range, 40-72 y). In the study group, mean values were as follows: plasma IL-8, 284.758 pg/mL (0-1000 pg/mL) plasma IL-10, 26.152 pg/mL (0-100 pg/mL) sCD27, 731.357 U/mL (139.9-1000 U/mL) white blood cell count, 59.9 x 10(9)/L (0.8-250.0 x 10(9)/L) hemoglobin count, 11.2 g/dL (5.0-16.2 g/dL) platelet count, 162.5 x 10(9)/L (29.8-317 x 10(9)/L) B(2) microglobulin (B(2)M) 3350.2 mg/L (274.7-7499.9 mg/L) CD38, 19.5% and lactate dehydrogenase (count, 497.5 U/L (263.0-1507 U/L). Patients represented all Rai stages, with 22.9% at stage 0, 11.4% at stage I, 11.4% at stage II, 41.4% at stage III, and 12.9% at stage IV. Plasma levels of IL-8, IL-10, and sCD27 were correlated between study and control groups; significantly higher IL-8 (P=.001) and sCD27 (P=.000) levels were found, but the IL-10 level was not significant (P=.139). Plasma IL-10 (P=.01) and sCD27 (P=.008) were positively correlated with Rai stage, but IL-8 was not (P=.146). Levels of sCD27 were significantly correlated with values for B2M (P=.000), hemoglobin (P=.028), lactate dehydrogenase (P=.001), CD19 (P=.03), and IL-10 (P=.000). IL-8 was significantly correlated with white blood cell (P=.000) count, and CD38 (P=.001) and CD5 (P=.006) levels. IL-10 was significantly correlated with B(2)M (P=.017), CD19 (P=.000), platelet (P=.002), and CD27 (P=.000). In survival distributions for CD27, IL-8 and IL-10 were found to have more significant relationships for all parameters (P=.0000). In conclusion, the authors suggest that sCD27, IL-8, and IL-10 are more significant prognostic factors for B-CLL when compared with others, and these values should correlate with new prognostic factors (eg, zeta-associated protein-70, mutated/unmutated immunoglobulin variable heavy chain).
本研究的调查人员探究了B细胞慢性淋巴细胞白血病（B-CLL）中可溶性CD27（sCD27）、白细胞介素（IL）-8和IL-10的水平，以及这些水平与疾病分期和预后的相关性。采用酶联免疫吸附测定法对22名健康供者和70例B-CLL患者（49例男性和21例女性）的血浆IL-8、IL-10和sCD27水平进行了评估。患者的平均年龄为61.57岁（范围44 - 75岁）。健康供者的平均年龄为62.09岁（范围40 - 72岁）。在研究组中，平均值如下：血浆IL-8为284.758 pg/mL（0 - 1000 pg/mL），血浆IL-10为26.152 pg/mL（0 - 100 pg/mL），sCD27为731.357 U/mL（139.9 - 1000 U/mL），白细胞计数为59.9×10⁹/L（0.8 - 250.0×10⁹/L），血红蛋白计数为11.2 g/dL（5.0 - 16.2 g/dL），血小板计数为162.5×10⁹/L（29.8 - 317×10⁹/L），β₂微球蛋白（β₂M）为3350.2 mg/L（274.7 - 7499.9 mg/L），CD38为19.5%，乳酸脱氢酶计数为497.5 U/L（263.0 - 1507 U/L）。患者涵盖了所有Rai分期，0期占22.9%，I期占11.4%，II期占11.4%，III期占41.4%，IV期占12.9%。研究组和对照组之间血浆IL-8、IL-10和sCD27水平具有相关性；发现IL-8（P = 0.001）和sCD27（P = 0.000）水平显著更高，但IL-10水平无统计学意义（P = 0.139）。血浆IL-10（P = 0.01）和sCD27（P = 0.008）与Rai分期呈正相关，但IL-8并非如此（P = 0.146）。sCD27水平与β₂M（P = 0.000）、血红蛋白（P = 0.028）、乳酸脱氢酶（P = 0.001）、CD19（P = 0.03）和IL-10（P = 0.000）的值显著相关。IL-8与白细胞计数（P = 0.000）、CD38（P = 0.001）和CD5（P = 0.006）水平显著相关。IL-10与β₂M（P = 0.017）、CD19（P = 0.000）、血小板（P = 0.002）和CD27（P = 0.000）显著相关。在CD27、IL-8和IL-10的生存分布中，发现所有参数之间的关系更为显著（P = 0.0000）。总之，作者认为与其他因素相比，sCD27、IL-8和IL-10是B-CLL更重要的预后因素，并且这些值应与新的预后因素（如ζ相关蛋白-70、突变/未突变的免疫球蛋白可变重链）相关。
