一种具有广泛特异性的脱水-N-乙酰胞壁酰-L-丙氨酸酰胺酶，锚定在大肠杆菌的外膜上。
An anhydro-N-acetylmuramyl-L-alanine amidase with broad specificity tethered to the outer membrane of Escherichia coli.
作者信息
Uehara Tsuyoshi, Park James T
机构信息
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA.
出版信息
J Bacteriol. 2007 Aug;189(15):5634-41. doi: 10.1128/JB.00446-07. Epub 2007 May 25.
Abstract
From its amino acid sequence homology with AmpD, we recognized YbjR, now renamed AmiD, as a possible second 1,6-anhydro-N-acetylmuramic acid (anhMurNAc)-l-alanine amidase in Escherichia coli. We have now confirmed that AmiD is an anhMurNAc-l-Ala amidase and demonstrated that AmpD and AmiD are the only enzymes present in E. coli that are able to cleave the anhMurNAc-l-Ala bond. The activity was present only in the outer membrane fraction obtained from an ampD mutant. In contrast to AmpD, which is specific for the anhMurNAc-l-alanine bond, AmiD also cleaved the bond between MurNAc and l-alanine in both muropeptides and murein sacculi. Unlike the periplasmic murein amidases, AmiD did not participate in cell separation. ampG mutants, which are unable to import GlcNAc-anhMurNAc-peptides into the cytoplasm, released mainly peptides into the medium due to AmiD activity, whereas an ampG amiD double mutant released a large amount of intact GlcNAc-anhMurNAc-peptides into the medium.
摘要
根据YbjR（现重命名为AmiD）与AmpD的氨基酸序列同源性，我们认为它可能是大肠杆菌中第二种1,6 - 脱水 - N - 乙酰胞壁酸（anhMurNAc）-L - 丙氨酸酰胺酶。我们现已证实AmiD是一种anhMurNAc - L - Ala酰胺酶，并证明AmpD和AmiD是大肠杆菌中仅有的能够切割anhMurNAc - L - Ala键的酶。该活性仅存在于从ampD突变体获得的外膜组分中。与对anhMurNAc - L - 丙氨酸键具有特异性的AmpD不同，AmiD还能切割多肽和胞壁质中MurNAc与L - 丙氨酸之间的键。与周质胞壁酰胺酶不同，AmiD不参与细胞分裂。ampG突变体无法将GlcNAc - anhMurNAc - 肽转运到细胞质中，由于AmiD的活性，其主要向培养基中释放肽，而ampG amiD双突变体则向培养基中释放大量完整的GlcNAc - anhMurNAc - 肽。
相似文献
1
An anhydro-N-acetylmuramyl-L-alanine amidase with broad specificity tethered to the outer membrane of Escherichia coli.一种具有广泛特异性的脱水-N-乙酰胞壁酰-L-丙氨酸酰胺酶，锚定在大肠杆菌的外膜上。
J Bacteriol. 2007 Aug;189(15):5634-41. doi: 10.1128/JB.00446-07. Epub 2007 May 25.
2
Specific structural features of the N-acetylmuramoyl-L-alanine amidase AmiD from Escherichia coli and mechanistic implications for enzymes of this family.大肠杆菌 N-乙酰胞壁酰-L-丙氨酸酰胺酶 AmiD 的特定结构特征及其对该家族酶的机制影响。
J Mol Biol. 2010 Mar 19;397(1):249-59. doi: 10.1016/j.jmb.2009.12.038. Epub 2009 Dec 28.
3
Substrate-induced inactivation of the Escherichia coli AmiD N-acetylmuramoyl-L-alanine amidase highlights a new strategy to inhibit this class of enzyme.底物诱导的大肠杆菌AmiD N-乙酰胞壁酰-L-丙氨酸酰胺酶失活突出了一种抑制这类酶的新策略。
Antimicrob Agents Chemother. 2009 Jul;53(7):2991-7. doi: 10.1128/AAC.01520-07. Epub 2009 Feb 23.
4
Bacterial cell wall recycling provides cytosolic muropeptides as effectors for beta-lactamase induction.细菌细胞壁循环利用可提供胞质内的胞壁肽作为诱导β-内酰胺酶的效应物。
EMBO J. 1994 Oct 3;13(19):4684-94. doi: 10.1002/j.1460-2075.1994.tb06792.x.
5
The N-acetyl-D-glucosamine kinase of Escherichia coli and its role in murein recycling.大肠杆菌的N-乙酰-D-葡萄糖胺激酶及其在胞壁质循环中的作用。
J Bacteriol. 2004 Nov;186(21):7273-9. doi: 10.1128/JB.186.21.7273-7279.2004.
6
Involvement of N-acetylmuramyl-L-alanine amidases in cell separation and antibiotic-induced autolysis of Escherichia coli.N-乙酰胞壁酰-L-丙氨酸酰胺酶在大肠杆菌细胞分离及抗生素诱导的自溶中的作用。
Mol Microbiol. 2001 Jul;41(1):167-78. doi: 10.1046/j.1365-2958.2001.02499.x.
7
Mutational analysis of the catalytic centre of the Citrobacter freundii AmpD N-acetylmuramyl-L-alanine amidase.弗氏柠檬酸杆菌AmpD N-乙酰胞壁酰-L-丙氨酸酰胺酶催化中心的突变分析
Biochem J. 2004 Jan 1;377(Pt 1):111-20. doi: 10.1042/BJ20030862.
8
Recycling of the anhydro-N-acetylmuramic acid derived from cell wall murein involves a two-step conversion to N-acetylglucosamine-phosphate.源自细胞壁胞壁质的脱水-N-乙酰胞壁酸的循环利用涉及两步转化为N-乙酰葡糖胺-磷酸的过程。
J Bacteriol. 2005 Jun;187(11):3643-9. doi: 10.1128/JB.187.11.3643-3649.2005.
9
N-acetylmuramoyl-L-alanine amidase of Escherichia coli K12. Possible physiological functions.大肠杆菌K12的N-乙酰胞壁酰-L-丙氨酸酰胺酶。可能的生理功能。
Eur J Biochem. 1983 Jun 15;133(2):371-7. doi: 10.1111/j.1432-1033.1983.tb07472.x.
10
The negative regulator of beta-lactamase induction AmpD is a N-acetyl-anhydromuramyl-L-alanine amidase.β-内酰胺酶诱导的负调节因子AmpD是一种N-乙酰脱氨胞壁酰-L-丙氨酸酰胺酶。
FEMS Microbiol Lett. 1994 Sep 15;122(1-2):159-64. doi: 10.1111/j.1574-6968.1994.tb07159.x.
引用本文的文献
1
Outer Membrane Vesicles: Biogenesis, Functions, and Issues.外膜囊泡：生物发生、功能和问题。
Microbiol Mol Biol Rev. 2022 Dec 21;86(4):e0003222. doi: 10.1128/mmbr.00032-22. Epub 2022 Sep 26.
2
Comparative Study of Bacterial SPOR Domains Identifies Functionally Important Differences in Glycan Binding Affinity.细菌 SPOR 结构域的比较研究鉴定了糖结合亲和力的功能重要差异。
J Bacteriol. 2022 Sep 20;204(9):e0025222. doi: 10.1128/jb.00252-22. Epub 2022 Aug 25.
3
Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli.转录阻遏蛋白 MurR 调控大肠杆菌细胞壁回收的分子基础。
Nucleic Acids Res. 2022 Jun 10;50(10):5948-5960. doi: 10.1093/nar/gkac442.
4
Induction of AmpC-Mediated β-Lactam Resistance Requires a Single Lytic Transglycosylase in Agrobacterium tumefaciens.诱导 AmpC 介导的β-内酰胺耐药性仅需在根瘤农杆菌中单一的溶菌转糖基酶。
Appl Environ Microbiol. 2022 Jun 28;88(12):e0033322. doi: 10.1128/aem.00333-22. Epub 2022 May 31.
5
Elucidation of host and symbiont contributions to peptidoglycan metabolism based on comparative genomics of eight aphid subfamilies and their Buchnera.基于 8 个蚜虫亚科及其共生菌 Buchnera 的比较基因组学阐明宿主和共生体对肽聚糖代谢的贡献。
PLoS Genet. 2022 May 6;18(5):e1010195. doi: 10.1371/journal.pgen.1010195. eCollection 2022 May.
6
Horizontal-Acquisition of a Promiscuous Peptidoglycan-Recycling Enzyme Enables Aphids To Influence Symbiont Cell Wall Metabolism.水平获得一种混杂的肽聚糖回收酶使蚜虫能够影响共生菌细胞壁代谢。
mBio. 2021 Dec 21;12(6):e0263621. doi: 10.1128/mBio.02636-21.
7
The extracellular vesicle generation paradox: a bacterial point of view.细胞外囊泡生成悖论：细菌的观点。
EMBO J. 2021 Nov 2;40(21):e108174. doi: 10.15252/embj.2021108174. Epub 2021 Oct 11.
8
Stable inheritance of Sinorhizobium meliloti cell growth polarity requires an FtsN-like protein and an amidase.苜蓿中华根瘤菌细胞生长极性的稳定遗传需要一种类似于 FtsN 的蛋白和一种酰胺酶。
Nat Commun. 2021 Jan 22;12(1):545. doi: 10.1038/s41467-020-20739-3.
9
Peptidoglycan: Structure, Synthesis, and Regulation.肽聚糖：结构、合成与调控。
EcoSal Plus. 2021 Jan;9(2). doi: 10.1128/ecosalplus.ESP-0010-2020.
10
Utilization of different MurNAc sources by the oral pathogen Tannerella forsythia and role of the inner membrane transporter AmpG.口腔病原体福赛坦纳菌对不同 MurNAc 来源的利用及内膜转运蛋白 AmpG 的作用。
BMC Microbiol. 2020 Nov 17;20(1):352. doi: 10.1186/s12866-020-02006-z.
本文引用的文献
1
Breaching the great wall: peptidoglycan and microbial interactions.突破“长城”：肽聚糖与微生物的相互作用
Nat Rev Microbiol. 2006 Sep;4(9):710-6. doi: 10.1038/nrmicro1486. Epub 2006 Aug 7.
2
MurQ Etherase is required by Escherichia coli in order to metabolize anhydro-N-acetylmuramic acid obtained either from the environment or from its own cell wall.大肠杆菌需要MurQ醚酶来代谢从环境或自身细胞壁获得的脱水N-乙酰胞壁酸。
J Bacteriol. 2006 Feb;188(4):1660-2. doi: 10.1128/JB.188.4.1660-1662.2006.
3
Scission of the lactyl ether bond of N-acetylmuramic acid by Escherichia coli "etherase".大肠杆菌“醚酶”对N - 乙酰胞壁酸乳酰醚键的裂解
J Biol Chem. 2005 Aug 26;280(34):30100-6. doi: 10.1074/jbc.M502208200. Epub 2005 Jun 27.
4
Recycling of the anhydro-N-acetylmuramic acid derived from cell wall murein involves a two-step conversion to N-acetylglucosamine-phosphate.源自细胞壁胞壁质的脱水-N-乙酰胞壁酸的循环利用涉及两步转化为N-乙酰葡糖胺-磷酸的过程。
J Bacteriol. 2005 Jun;187(11):3643-9. doi: 10.1128/JB.187.11.3643-3649.2005.
5
Microbial factor-mediated development in a host-bacterial mutualism.宿主-细菌共生关系中微生物因子介导的发育
Science. 2004 Nov 12;306(5699):1186-8. doi: 10.1126/science.1102218.
6
The N-acetyl-D-glucosamine kinase of Escherichia coli and its role in murein recycling.大肠杆菌的N-乙酰-D-葡萄糖胺激酶及其在胞壁质循环中的作用。
J Bacteriol. 2004 Nov;186(21):7273-9. doi: 10.1128/JB.186.21.7273-7279.2004.
7
Sorting of lipoproteins to the outer membrane in E. coli.脂蛋白在大肠杆菌外膜中的分选
Biochim Biophys Acta. 2004 Jul 23;1693(1):5-13. doi: 10.1016/j.bbamcr.2004.02.005.
8
Mutational analysis of the catalytic centre of the Citrobacter freundii AmpD N-acetylmuramyl-L-alanine amidase.弗氏柠檬酸杆菌AmpD N-乙酰胞壁酰-L-丙氨酸酰胺酶催化中心的突变分析
Biochem J. 2004 Jan 1;377(Pt 1):111-20. doi: 10.1042/BJ20030862.
9
The pair of arginine codons AGA AGG close to the initiation codon of the lambda int gene inhibits cell growth and protein synthesis by accumulating peptidyl-tRNAArg4.靠近λ整合酶基因起始密码子的精氨酸密码子对AGA、AGG，通过积累肽基-tRNAArg4抑制细胞生长和蛋白质合成。
Mol Microbiol. 2003 Aug;49(4):1043-9. doi: 10.1046/j.1365-2958.2003.03611.x.
10
Prediction of lipoprotein signal peptides in Gram-negative bacteria.革兰氏阴性菌中脂蛋白信号肽的预测
Protein Sci. 2003 Aug;12(8):1652-62. doi: 10.1110/ps.0303703.
Zotero 插件