Kuncharoen Nattakorn, Techo Sujitra, Savarajara Ancharida, Tanasupawat Somboon
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
Mahidol University, Nakhonsawan Campus, Nakhonsawan, Thailand.
Mycology. 2020 Mar 26;11(4):279-286. doi: 10.1080/21501203.2020.1745922.
Thirty-three yeasts were isolated from palm oil industrial wastes and traditional fermented foods in Thailand. Based on the analysis of the sequences of the D1/D2 region of the large subunit ribosomal RNA gene (LSU rDNA) and their phenotypic characteristics, they were identified as (11 isolates), (1 isolate), (2 isolates), (1 isolate), (1 isolate), (1 isolate), (2 isolates), (5 isolates), (2 isolates), (1 isolate), and (3 isolates), (1 isolate), and (2 isolates). Seven strains, 4E-1D, 5E-1T and 5E-2D, 8E-1T and 8E-1D, and Fy-12 and Fy-13, showed high lipolytic activity ranged from 5.21 ± 0.09 to 45.68 ± 2.37 U/mL. Moreover, these seven strains exhibited good lipolytic activity after culturing in the medium containing palm oil (11.79 ± 0.67 to 28.19 ± 4.84 U/mL) and soy oil (9.14 ± 1.08 to 22.97 ± 0.69 U/mL) as lipase inducers. The result of this study suggests that the palm oil industrial wastes and Thai fermented foods could be promised as the invaluable sources of lipolytic yeasts.
从泰国的棕榈油工业废料和传统发酵食品中分离出了33株酵母。基于对大亚基核糖体RNA基因（LSU rDNA）D1/D2区域序列及其表型特征的分析，它们被鉴定为（11株分离株）、（1株分离株）、（2株分离株）、（1株分离株）、（1株分离株）、（1株分离株）、（2株分离株）、（5株分离株）、（2株分离株）、（1株分离株）、（3株分离株）、（1株分离株）和（2株分离株）。七株菌株，4E-1D、5E-1T和5E-2D、8E-1T和8E-1D、Fy-12和Fy-13，表现出较高的脂肪分解活性，范围为5.21±0.09至45.68±2.37 U/mL。此外，在含有棕榈油（11.79±0.67至28.19±4.84 U/mL）和大豆油（9.14±1.08至22.97±0.69 U/mL）作为脂肪酶诱导剂的培养基中培养后，这七株菌株表现出良好的脂肪分解活性。本研究结果表明，棕榈油工业废料和泰国发酵食品有望成为脂肪分解酵母的宝贵来源。
