Savitch Leonid V, Subramaniam Rajagopal, Allard Ghislaine C, Singh Jas
Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, ONT, Canada.
Biochem Biophys Res Commun. 2007 Jul 27;359(2):234-8. doi: 10.1016/j.bbrc.2007.05.084. Epub 2007 May 24.
Overexpression (OE) was used to study the role of the Arabidopsis Golden2-like (GLK1) transcriptional activator in regulating gene expression. Affymetrix Gene Chip and RT-PCR analyses indicated that GLK1 OE in Arabidopsis reprogrammed gene expression networks to enhance a high constitutive expression of genes encoding disease defense related proteins. These include PR10, isochorismate synthase, antimicrobial peptides, glycosyl hydrolases, MATE efflux and other genes associated with pathogen response and detoxification. However, PR1, an indicator of systemic acquired resistance (SAR), was downregulated in GLK1 OE. GLK1 OE in Arabidopsis confers resistance to Fusarium graminearum, a broad host pathogen responsible for major losses in cereal crops. This is the first identification of the GLK1 'regulon' and a novel role for GLK1 in plant defense, suggesting its potential use for providing disease resistance in crop plants.
过表达（OE）被用于研究拟南芥类Golden2（GLK1）转录激活因子在调控基因表达中的作用。Affymetrix基因芯片和逆转录聚合酶链反应（RT-PCR）分析表明，拟南芥中GLK1的过表达重新编程了基因表达网络，以增强编码疾病防御相关蛋白的基因的高组成型表达。这些基因包括PR10、异分支酸合酶、抗菌肽、糖基水解酶、多药和有毒化合物排出家族（MATE）转运蛋白以及其他与病原体应答和解毒相关的基因。然而，作为系统获得性抗性（SAR）指标的PR1在GLK1过表达植株中表达下调。拟南芥中GLK1的过表达赋予了对禾谷镰刀菌的抗性，禾谷镰刀菌是一种广泛寄主的病原体，会导致谷类作物遭受重大损失。这是首次鉴定出GLK1的“调控子”以及GLK1在植物防御中的新作用，表明其在为作物提供抗病性方面具有潜在用途。
