益肾清火方对实验性牙周炎大鼠牙周炎症及免疫的影响

[Effect of yishenqinghuo recipe on periodontal inflammation and immunity of rats with experimental periodontitis].

作者信息

Luo Li-jun, Shu Rong, Song Zhong-chen, Wu An-ping, Yu Jin, Shi Gui-ying

机构信息

Department of Oral Medicine, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.

出版信息

Shanghai Kou Qiang Yi Xue. 2006 Dec;15(6):605-9.

PMID:17533712

Abstract

PURPOSE

To evaluate the effect of Yishenqinghuo recipe on periodontal inflammation and immunity of rats with experimental periodontitis.

METHODS

12 months old Spague-Dawley rats were used in this study. 78 rats were randomly divided into 4 groups. group A: control group (with no periodontitis, fed with the same dosage of saline as group D); group B: model group (with periodontitis, fed with the same dosage of saline as group D); group C: high dosage group (with periodontitis, fed with double dosage of medicine as group D); group D: equivalent dosage group (with periodontitis, fed with clinical equivalent effective dosage of medicine). After being gavaged with medicine/saline for 3 months, the periodontium was analyzed through histological slices; and the amount of CD4+T,CD8+T, the ratio of CD4+T/CD8+T, IL-2 and IL-1beta in peripheral blood were detected by flow cytometry and ELISA. All the results were analyzed by ANOVA, with the use of SAS 6.04 software package.

RESULTS

It was found that the periodontal inflammation of group C and D were improved significantly; the ratio of CD4+T/CD8+T in peripheral blood for this 4 groups were 3.55 +/- 0.94, 2.42 +/- 0.75, 3.23 +/- 1.14 and 3.29 +/- 0.83; the level of IL-2 and IL-1beta were (36.03+/- 2.63/179.04 +/- 17.29) pg/ml, (25.18 +/- 3.08/306.09 +/- 13.38) pg/ml, (38.44 +/- 2.58/176.33 +/- 45.38) pg/ml and (36.81 +/- 2.45/182.13 +/- 43.97) pg/ml. Compared with group B, the ratio of CD4+T/CD8+T for group C and D was significantly rised (P < 1.05); the level of IL-2 increased significantlyand IL-1beta decreased accordingly (P < 0.01). However, there was no significant difference between group C and D (P > 0.05).

CONCLUSION

From this study, we conclude that Yishenqinghuo recipe can improve the periodontal inflammation and adjust the immunity of rats with experimental periodontitis. Supported by National "Tenth Five-Year" Key Science and Technology PROject (Grant No. 2004BA720A26) and Natural Science Foundation of Shanghai Municipality (Grant No. 03Zr14081).

摘要

目的

评价益肾清火方对实验性牙周炎大鼠牙周组织炎症及免疫功能的影响。

方法

选用12月龄Spague-Dawley大鼠，78只大鼠随机分为4组。A组：对照组（无牙周炎，给予与D组等量的生理盐水）；B组：模型组（有牙周炎，给予与D组等量的生理盐水）；C组：高剂量组（有牙周炎，给予D组两倍剂量的药物）；D组：等效剂量组（有牙周炎，给予临床等效有效剂量的药物）。给药/生理盐水灌胃3个月后，通过组织切片分析牙周组织；采用流式细胞术和ELISA检测外周血中CD4+T、CD8+T细胞数量、CD4+T/CD8+T细胞比值、IL-2和IL-1β水平。所有结果采用ANOVA分析，使用SAS 6.04软件包。

结果

发现C组和D组的牙周炎症明显改善；4组外周血中CD4+T/CD8+T细胞比值分别为3.55±0.94、2.42±0.75、3.23±1.14和3.29±0.83；IL-2和IL-1β水平分别为(36.03±2.63/179.04±17.29)pg/ml、(25.18±3.08/306.09±13.38)pg/ml、(38.44±2.58/176.33±45.38)pg/ml和(36.81±2.45/182.13±43.97)pg/ml。与B组相比，C组和D组的CD4+T/CD8+T细胞比值显著升高（P<0.05）；IL-2水平显著升高，IL-1β水平相应降低（P<0.01）。然而，C组和D组之间无显著差异（P>0.05）。