Silva R M G, Matera J M, Ribeiro A A C M
Department of Surgery, College of Veterinary Medicine, University of São Paulo, Sao Paulo, Brazil.
Anat Histol Embryol. 2007 Jun;36(3):220-4. doi: 10.1111/j.1439-0264.2007.00759.x.
Due to a raised ethical mentality, veterinary schools are pursuing methods to preserve animal corpses used for surgical technique classes in an attempt to reduce the use of living animals for teaching. Generally speaking, animal and human bodies are usually preserved with 10% aqueous formalin solution especially for descriptive anatomy classes. Other possibilities include the use of glycerol, alcohol and phenol. At present, new fixatives have been developed to allow a better and longer preservation of animal corpses in order to maintain organoleptic characteristics, i.e. colour, texture, as close as possible to what students will deal with living animals. From 2004, in our college, surgical technique classes no longer use living animals for students' training. Instead, canine corpses chemically preserved with modified Larssen (MLS) and Laskowski (LS) solutions are preferred. The purpose of this study was to investigate comparatively the biological quality of preservation of these two solutions and to evaluate students' learning and acceptance of this new teaching method. Although these fixatives maintain body flexibility, LS solution failed to keep an ordinary tissue colouration (cadavers were intensely red) and tissue preservation was not adequate. By contrast, MLS solution, however, did not alter the colouration of cadavers which was fairly similar to that normally found in living animals. A remarkable characteristic was a very strong and unpleasant sugary odour in LS-preserved animals and therefore the MLS solution was the elected method to preserve cadavers for surgical technique classes. The students' feedback to the use of Larssen-preserved cadavers was very satisfactory, i.e. 96.6% of students were in favour of the use of cadavers for surgical training and on average 91.8% (2002-2003) of students preferred the MLS solution as the chemical preserver, whereas only 8.2% elected LS solution for teaching purposes. From the students' point of view (95.1%) the ideal class would be an initial training in MLS cadavers followed by classes with animals admitted to the Veterinary Hospital.
由于道德观念的提升,兽医学院正在寻求保存用于外科技术课程的动物尸体的方法,以减少用于教学的活体动物的使用。一般来说,动物和人体通常用10%的甲醛水溶液保存,特别是用于描述性解剖学课程。其他可能性包括使用甘油、酒精和苯酚。目前,已经开发出了新的固定剂,以便更好、更长久地保存动物尸体,以保持感官特性,即颜色、质地,尽可能接近学生在处理活体动物时所看到的。从2004年起,在我们学院,外科技术课程不再使用活体动物进行学生培训。相反,用改良的拉尔森(MLS)溶液和拉斯科夫斯基(LS)溶液化学保存的犬类尸体更受青睐。本研究的目的是比较这两种溶液的生物保存质量,并评估学生对这种新教学方法的学习和接受程度。尽管这些固定剂能保持尸体的柔韧性,但LS溶液未能保持正常的组织颜色(尸体呈深红色),组织保存也不充分。相比之下,MLS溶液却没有改变尸体的颜色,与活体动物通常的颜色相当相似。一个显著的特点是,用LS溶液保存的动物有非常强烈且难闻的甜味,因此MLS溶液是用于外科技术课程尸体保存的首选方法。学生对使用拉尔森溶液保存的尸体的反馈非常令人满意,即96.6%的学生赞成使用尸体进行外科训练,平均91.8%(2002 - 2003年)的学生更喜欢MLS溶液作为化学防腐剂,而只有8.2%的学生选择LS溶液用于教学目的。从学生的角度来看(95.1%),理想的课程应该是先在MLS溶液保存的尸体上进行初始培训,然后再进行使用进入兽医医院的动物的课程。