Giordano Alessandra, Varesi Paola, Bertini Alessia, Villa Laura, Dionisi Anna Maria, Venditti Mario, Carfagna Paolo, Luzzi Ida, Mancini Carlo, Carattoli Alessandra
Department of Science and Public Health, Microbiology Unit, University of Rome La Sapienza, Rome, Italy.
Microb Drug Resist. 2007 Spring;13(1):37-43. doi: 10.1089/mdr.2006.9995.
In this study 45 epidemic and sporadic isolates of Acinetobacter baumannii were investigated by antimicrobial resistance, integron identifications and genotyping. Isolates were genotyped by random amplified polymorphism (RAPD) DNA and pulsed-field gel electrophoresis (PFGE). Four different RAPD patterns were observed among the isolates of our collection, further discerned in six PFGE types. Two prevalent genotypes were identified, one corresponding to a carbapenem resistant epidemic clone, causing an outbreak at the intensive care unit of a hospital of Rome. Two class 1 integrons, carrying different gene cassette arrays, were identified among the two prevalent genotypes. Nucleotide analysis of the integron-variable regions revealed the presence of the aacA4, orfO, bla(OXA-20), and aacC1, orfX, orfX', aadA1 gene cassette arrays, respectively. All the carbapenem resistant strains analyzed in this study carried the bla (OXA-58) gene located on plasmids.
在本研究中，通过抗菌药物耐药性、整合子鉴定和基因分型对45株鲍曼不动杆菌的流行株和散发株进行了调查。采用随机扩增多态性（RAPD）DNA和脉冲场凝胶电泳（PFGE）对分离株进行基因分型。在我们收集的分离株中观察到四种不同的RAPD模式，进一步细分为六种PFGE类型。鉴定出两种流行基因型，一种对应于耐碳青霉烯类的流行克隆，在罗马一家医院的重症监护病房引起了一次暴发。在两种流行基因型中鉴定出两个携带不同基因盒阵列的1类整合子。对整合子可变区的核苷酸分析分别揭示了aacA4、orfO、bla(OXA-20)，以及aacC1、orfX、orfX'、aadA1基因盒阵列的存在。本研究中分析的所有耐碳青霉烯类菌株均携带位于质粒上的bla (OXA-58)基因。
