[高效液相色谱法测定不同炮制品巴戟天中水晶兰苷的含量]
[Determination of monotropein in Radix Morindae from different processed products by HPLC].
作者信息
Xu Ji-yin, Liang Ying-jiao, Ding Ping
机构信息
College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou 510405, China.
出版信息
Zhong Yao Cai. 2007 Jan;30(1):20-2.
OBJECTIVE
To determine the contents of monotropein in the different processed Products of Radix Morindae.
CONDITIONS
Kromasil C18 (150 mm x 4. 6 mm,5 micorm) column; The mobile phase was methol-0.4% phosphate solution (5:95-->28.8: 71.2, 15 min); The velocity of flow was 1 ml/min; The detection wavelength was at 210 nm; And column temperature was 25 degrees C RESULTS: There were obvious differences between the crude and kinds of different processed products of monotropein in the Radix Morindae, The contents of monotropein from the different processed products (crude drugs, removed from wood, processed with salt, prepared) were 13.92 mg/g,9.10 mg/g, 9.21 mg/g, 12.86 mg/g, respectively.
CONCLUSION
The different processing methods can make the contents of monotropein in the Radix Morindae.
目的
测定不同炮制方法的巴戟天中水晶兰苷的含量。
条件
Kromasil C18柱（150 mm×4.6 mm，5μm）；流动相为甲醇-0.4%磷酸溶液（5:95→28.8:71.2，15分钟）；流速为1 ml/min；检测波长为210 nm；柱温为25℃。结果：巴戟天生品与不同炮制品中水晶兰苷含量有明显差异，不同炮制品（生品、去木、盐制、制）中水晶兰苷含量分别为13.92 mg/g、9.10 mg/g、9.21 mg/g、12.86 mg/g。
结论
不同炮制方法可使巴戟天中水晶兰苷的含量发生变化。
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