Paz Beatriz, Daranas Antonio H, Cruz Patricia G, Franco José M, Napolitano José G, Norte Manuel, Fernández José J
Instituto de Investigaciones Marinas (CSIC), Eduardo Cabello 6, 36080 Vigo, Spain.
Toxicon. 2007 Sep 15;50(4):470-8. doi: 10.1016/j.toxicon.2007.04.011. Epub 2007 May 1.
The main toxins produced by the dinoflagellate Prorocentrum belizeanum, DTX5c and 7-hydroxymethyl-2methylene-octa-4,7 dienyl okadaate, were studied by liquid chromatography-coupled with ion trap mass spectrometry (LC-MS/MS). Their retention times (RTs) and fragmentation patterns were established, in particular those of DTX5c, as there is a lack of data about these water soluble OA sulphated derivatives. As an application of the LC-MS methodology, a sample of the toxic P. belizeanum culture was analysed for these metabolites. Both metabolites were detected in cells as majority compounds, whereas levels of these compounds in the culture media were undetectable.
利用液相色谱-离子阱质谱联用技术（LC-MS/MS）对来自伯利兹原甲藻（Prorocentrum belizeanum）的主要毒素DTX5c和7-羟甲基-2-亚甲基-辛-4,7-二烯基冈田酸酯进行了研究。确定了它们的保留时间（RTs）和碎裂模式，特别是DTX5c的，因为关于这些水溶性OA硫酸化衍生物的数据较少。作为LC-MS方法的应用，对有毒的伯利兹原甲藻培养物样品进行了这些代谢物的分析。两种代谢物在细胞中作为主要化合物被检测到，而在培养基中这些化合物的水平则无法检测到。
