Paz Beatriz, Daranas Antonio H, Cruz Patricia G, Franco José M, Pizarro Gemita, Souto María L, Norte Manuel, Fernández José J
Instituto de Investigaciones Marinas (CSIC), Eduardo Cabello 6, 36080 Vigo, Spain.
Toxicon. 2007 Aug;50(2):225-35. doi: 10.1016/j.toxicon.2007.03.015. Epub 2007 Mar 28.
In the Diarrhetic Shellfish Poisoning (DSP) phenomena, the parent toxins, namely okadaic acid (OA) and/or dinophysistoxin-2 (DTX2), are predominantly found esterified. Therefore, a toxicity assessment of a sample can only be performed after an alkaline hydrolysis step in order to recover the parent molecules in their free form. The presence of several OA diol esters has already been confirmed in Prorocentrum lima and Prorocentrum belizeanum cultures. This paper reports on the analysis of OA diol esters using liquid chromatography coupled with mass spectrometry (LC-MS/MS), and establishes a method for their detection and identification based upon their retention times (RT) and the fragmentation patterns of their mass spectra.
在腹泻性贝类中毒（DSP）现象中，母体毒素，即冈田酸（OA）和/或鳍藻毒素-2（DTX2），主要以酯化形式存在。因此，为了以游离形式回收母体分子，只能在碱性水解步骤之后对样品进行毒性评估。在利马原甲藻和伯利兹原甲藻培养物中已经证实存在几种OA二醇酯。本文报道了使用液相色谱-质谱联用（LC-MS/MS）对OA二醇酯进行分析，并基于其保留时间（RT）和质谱碎裂模式建立了一种检测和鉴定它们的方法。
