通过液相色谱-质谱检测鉴定一种新型腹泻性贝类中毒毒素——19-表冈田酸。

Identification of 19-epi-okadaic acid, a new diarrhetic shellfish poisoning toxin, by liquid chromatography with mass spectrometry detection.

作者信息

Paz Beatriz, Daranas Antonio H, Cruz Patricia G, Franco José M, Norte Manuel, Fernández José J

机构信息

Fitoplancton Tóxico, Instituto de Investigaciones Marinas (CSIC), Eduardo Cabello 6, 36080 Vigo, Spain.

出版信息

Mar Drugs. 2008;6(3):489-95. doi: 10.3390/md20080024. Epub 2008 Aug 28.

DOI:10.3390/md20080024

PMID:19005581

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2579738/

Abstract

Okadaic acid (1) (OA) and its congeners are mainly responsible for diarrhetic shellfish poisoning (DSP) syndrome. The presence of several OA derivatives have already been confirmed in Prorocentrum and Dinophysis spp. In this paper, we report on the detection and identification of a new DSP toxin, the OA isomer 19-epi-okadaic acid (2) (19-epi-OA), isolated from cultures of Prorocentrum belizeanum, by determining its retention time (RT) and fragmentation pattern using liquid chromatography coupled with mass spectrometry (LC-MS/MS).

摘要

冈田酸（1）（OA）及其同系物是引发腹泻性贝类中毒（DSP）综合征的主要原因。在原甲藻属和鳍藻属物种中已证实存在多种OA衍生物。在本文中，我们报告了一种新的DSP毒素——OA异构体19-表冈田酸（2）（19-epi-OA）的检测与鉴定，该毒素是从伯利兹原甲藻培养物中分离得到的，通过液相色谱-质谱联用（LC-MS/MS）测定其保留时间（RT）和碎片模式来实现。