蜡样芽孢杆菌孢子的萌发是由来自分化的Caco-2细胞（一种模拟小肠上皮细胞的人类细胞系）的萌发剂诱导的。
Germination of Bacillus cereus spores is induced by germinants from differentiated Caco-2 Cells, a human cell line mimicking the epithelial cells of the small intestine.
作者信息
Wijnands L M, Dufrenne J B, van Leusden F M, Abee T
机构信息
Laboratory for Zoonoses and Environmental Microbiology (LZO), National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.
出版信息
Appl Environ Microbiol. 2007 Aug;73(15):5052-4. doi: 10.1128/AEM.02390-06. Epub 2007 Jun 1.
Abstract
Spores of 11 enterotoxigenic strains of Bacillus cereus isolated from foods and humans adhered with similar efficiencies to Caco-2 cells, whereas subsequent germination triggering was observed with only 8 of these strains. Notably, Hep-2 cells did not trigger germination, while spores of all strains displayed similar germination efficiencies in brain heart infusion broth.
摘要
从食品和人体中分离出的11株产肠毒素蜡样芽孢杆菌菌株的孢子以相似的效率黏附于Caco-2细胞，然而随后仅观察到其中8株菌株的孢子萌发触发。值得注意的是，Hep-2细胞不会触发孢子萌发，而所有菌株的孢子在脑心浸液肉汤中显示出相似的萌发效率。
相似文献
1
Germination of Bacillus cereus spores is induced by germinants from differentiated Caco-2 Cells, a human cell line mimicking the epithelial cells of the small intestine.蜡样芽孢杆菌孢子的萌发是由来自分化的Caco-2细胞（一种模拟小肠上皮细胞的人类细胞系）的萌发剂诱导的。
Appl Environ Microbiol. 2007 Aug;73(15):5052-4. doi: 10.1128/AEM.02390-06. Epub 2007 Jun 1.
2
Role of germinant receptors in Caco-2 cell-initiated germination of Bacillus cereus ATCC 14579 endospores.萌发受体在蜡样芽孢杆菌ATCC 14579芽孢的Caco-2细胞启动萌发中的作用。
Appl Environ Microbiol. 2009 Feb;75(4):1201-3. doi: 10.1128/AEM.02238-08. Epub 2008 Dec 16.
3
Germination of Bacillus cereus spores adhered to stainless steel.附着在不锈钢上的蜡样芽孢杆菌孢子的萌发
Int J Food Microbiol. 2007 May 30;116(3):367-71. doi: 10.1016/j.ijfoodmicro.2007.02.012. Epub 2007 Mar 6.
4
The adhesion of Bacillus cereus spores to epithelial cells might be an additional virulence mechanism.蜡样芽孢杆菌孢子与上皮细胞的黏附可能是另一种致病机制。
Int J Food Microbiol. 1998 Jan 6;39(1-2):93-9. doi: 10.1016/s0168-1605(97)00121-9.
5
Effects of moderately high pressure plus heat on the germination and inactivation of Bacillus cereus spores lacking proteins involved in germination.适度高压联合加热对缺乏参与萌发的蛋白质的蜡样芽孢杆菌孢子萌发和失活的影响
Lett Appl Microbiol. 2009 Nov;49(5):646-51. doi: 10.1111/j.1472-765X.2009.02721.x. Epub 2009 Aug 22.
6
Spores from mesophilic Bacillus cereus strains germinate better and grow faster in simulated gastro-intestinal conditions than spores from psychrotrophic strains.嗜温蜡样芽孢杆菌菌株的孢子在模拟胃肠道条件下比嗜冷菌株的孢子发芽更好且生长更快。
Int J Food Microbiol. 2006 Nov 1;112(2):120-8. doi: 10.1016/j.ijfoodmicro.2006.06.015. Epub 2006 Jul 24.
7
Analysis of Germination Capacity and Germinant Receptor (Sub)clusters of Genome-Sequenced Bacillus cereus Environmental Isolates and Model Strains.基因组测序的蜡样芽孢杆菌环境分离株和模式菌株的发芽能力及发芽受体（亚）簇分析
Appl Environ Microbiol. 2017 Feb 1;83(4). doi: 10.1128/AEM.02490-16. Print 2017 Feb 15.
8
Effects of porcine bile on survival of Bacillus cereus vegetative cells and Haemolysin BL enterotoxin production in reconstituted human small intestine media.猪胆汁对蜡样芽孢杆菌营养细胞存活及重组人小肠培养基中溶血素BL肠毒素产生的影响。
J Appl Microbiol. 2007 Nov;103(5):1568-75. doi: 10.1111/j.1365-2672.2007.03410.x.
9
Kefiran antagonizes cytopathic effects of Bacillus cereus extracellular factors.凯夫兰拮抗蜡样芽孢杆菌细胞外因子的细胞病变效应。
Int J Food Microbiol. 2008 Feb 29;122(1-2):1-7. doi: 10.1016/j.ijfoodmicro.2007.11.046. Epub 2007 Nov 28.
10
Kefiran protects Caco-2 cells from cytopathic effects induced by Bacillus cereus infection.开菲尔多糖可保护Caco-2细胞免受蜡样芽孢杆菌感染诱导的细胞病变效应。
Antonie Van Leeuwenhoek. 2009 Nov;96(4):505-13. doi: 10.1007/s10482-009-9366-z. Epub 2009 Jul 25.
引用本文的文献
1
Enigmatic Pilus-Like Endospore Appendages of Group Species.具有神秘性的类群种芽孢杆菌样内生孢子附属物。
Int J Mol Sci. 2021 Nov 16;22(22):12367. doi: 10.3390/ijms222212367.
2
The Food Poisoning Toxins of .《. 的食物中毒毒素》
Toxins (Basel). 2021 Jan 28;13(2):98. doi: 10.3390/toxins13020098.
3
The Food Infection as Multifactorial Process.食品感染是一个多因素过程。
Toxins (Basel). 2020 Nov 5;12(11):701. doi: 10.3390/toxins12110701.
4
Enteropathogenic Potential of Isolates from Soil, Animals, Food and Biopesticides.来自土壤、动物、食物和生物农药的分离株的肠道致病潜力。
Foods. 2020 Oct 17;9(10):1484. doi: 10.3390/foods9101484.
5
Risk presented to minimally processed chilled foods by psychrotrophic .嗜冷微生物给轻度加工冷藏食品带来的风险。
Trends Food Sci Technol. 2019 Nov;93:94-105. doi: 10.1016/j.tifs.2019.08.024.
6
Porcine Gastric Mucin Triggers Toxin Production of Enteropathogenic .猪胃黏液触发肠致病性
Infect Immun. 2019 Mar 25;87(4). doi: 10.1128/IAI.00765-18. Print 2019 Apr.
7
Consumed Foodstuffs Have a Crucial Impact on the Toxic Activity of Enteropathogenic .食用的食物对肠道致病菌的毒性活性有至关重要的影响。
Front Microbiol. 2018 Aug 17;9:1946. doi: 10.3389/fmicb.2018.01946. eCollection 2018.
8
Simulating Intestinal Growth Conditions Enhances Toxin Production of Enteropathogenic .模拟肠道生长条件可增强肠道致病菌的毒素产生。
Front Microbiol. 2017 Apr 12;8:627. doi: 10.3389/fmicb.2017.00627. eCollection 2017.
9
Bacillus cereus NVH 0500/00 Can Adhere to Mucin but Cannot Produce Enterotoxins during Gastrointestinal Simulation.蜡样芽孢杆菌NVH 0500/00在胃肠道模拟过程中可黏附黏蛋白，但不能产生肠毒素。
Appl Environ Microbiol. 2015 Oct 23;82(1):289-96. doi: 10.1128/AEM.02940-15. Print 2016 Jan 1.
10
Survival and germination of Bacillus cereus spores without outgrowth or enterotoxin production during in vitro simulation of gastrointestinal transit.在胃肠道转运的体外模拟过程中，蜡样芽胞杆菌孢子在不生长或不产生肠毒素的情况下的存活和发芽。
Appl Environ Microbiol. 2012 Nov;78(21):7698-705. doi: 10.1128/AEM.02142-12. Epub 2012 Aug 24.
本文引用的文献
1
Spores from mesophilic Bacillus cereus strains germinate better and grow faster in simulated gastro-intestinal conditions than spores from psychrotrophic strains.嗜温蜡样芽孢杆菌菌株的孢子在模拟胃肠道条件下比嗜冷菌株的孢子发芽更好且生长更快。
Int J Food Microbiol. 2006 Nov 1;112(2):120-8. doi: 10.1016/j.ijfoodmicro.2006.06.015. Epub 2006 Jul 24.
2
Characterization of germination receptors of Bacillus cereus ATCC 14579.蜡样芽孢杆菌ATCC 14579发芽受体的特性分析
Appl Environ Microbiol. 2006 Jan;72(1):44-53. doi: 10.1128/AEM.72.1.44-53.2006.
3
gerR, a novel ger operon involved in L-alanine- and inosine-initiated germination of Bacillus cereus ATCC 14579.gerR，一个参与蜡样芽孢杆菌ATCC 14579由L-丙氨酸和肌苷引发的芽孢萌发的新型ger操纵子。
Appl Environ Microbiol. 2005 Feb;71(2):774-81. doi: 10.1128/AEM.71.2.774-781.2005.
4
Spore germination.孢子萌发
Curr Opin Microbiol. 2003 Dec;6(6):550-6. doi: 10.1016/j.mib.2003.10.001.
5
Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis.蜡样芽孢杆菌的基因组序列及与炭疽芽孢杆菌的比较分析。
Nature. 2003 May 1;423(6935):87-91. doi: 10.1038/nature01582.
6
Germination of Bacillus cereus spores in response to L-alanine and to inosine: the roles of gerL and gerQ operons.蜡样芽孢杆菌孢子对L-丙氨酸和肌苷的萌发：gerL和gerQ操纵子的作用。
Microbiology (Reading). 2002 Jul;148(Pt 7):2089-2095. doi: 10.1099/00221287-148-7-2089.
7
Semiautomated metabolic staining assay for Bacillus cereus emetic toxin.蜡样芽孢杆菌呕吐毒素的半自动代谢染色测定法
Appl Environ Microbiol. 1999 Apr;65(4):1811-2. doi: 10.1128/AEM.65.4.1811-1812.1999.
8
Surface protein p104 is involved in adhesion of Listeria monocytogenes to human intestinal cell line, Caco-2.表面蛋白p104参与单核细胞增生李斯特菌与人肠道细胞系Caco-2的黏附。
J Med Microbiol. 1999 Feb;48(2):117-124. doi: 10.1099/00222615-48-2-117.
9
The adhesion of Bacillus cereus spores to epithelial cells might be an additional virulence mechanism.蜡样芽孢杆菌孢子与上皮细胞的黏附可能是另一种致病机制。
Int J Food Microbiol. 1998 Jan 6;39(1-2):93-9. doi: 10.1016/s0168-1605(97)00121-9.
10
Functional relationships between L- and D-alanine, inosine and NH4Cl during germination of spores of Bacillus cereus T.蜡样芽孢杆菌T型芽孢萌发过程中L-丙氨酸、D-丙氨酸、肌苷和氯化铵之间的功能关系
J Gen Microbiol. 1988 Nov;134(11):3001-10. doi: 10.1099/00221287-134-11-3001.
Zotero 插件