Meyer Andreas, Pellaux Rene, Panke Sven
Bioprocess Laboratory, ETH Zurich, Universitaetsstrasse 6, 8092 Zurich, Switzerland.
Curr Opin Microbiol. 2007 Jun;10(3):246-53. doi: 10.1016/j.mib.2007.05.009. Epub 2007 Jun 4.
Huge numbers of enzymes have evolved in nature to function in aqueous environments at moderate temperatures and neutral pH. This gives us, in principle, the unique opportunity to construct multistep reaction systems of considerable catalytic complexity in vitro. However, this opportunity is rarely exploited beyond research scale, because such systems are difficult to assemble and to operate productively. Recent advances in DNA synthesis, genome engineering, high-throughput analytics, model-based analysis of biochemical systems and (semi-)rational protein engineering suggest that we have all the tools available to rationally design and efficiently operate such systems of enzymes, and finally harvest their potential for preparative syntheses.
自然界中已经进化出大量的酶，以在适度温度和中性pH值的水环境中发挥作用。原则上，这为我们提供了一个独特的机会，在体外构建具有相当催化复杂性的多步反应系统。然而，除了研究规模之外，这种机会很少被利用，因为这样的系统难以组装且难以高效运行。DNA合成、基因组工程、高通量分析、基于模型的生化系统分析以及（半）理性蛋白质工程的最新进展表明，我们拥有所有可用的工具来合理设计并高效运行此类酶系统，并最终挖掘其在制备合成方面的潜力。
