Soukka T, Lumikari M, Tenovuo J
Department of Cariology, University of Turku, Finland.
Scand J Dent Res. 1991 Oct;99(5):390-6. doi: 10.1111/j.1600-0722.1991.tb01046.x.
We have studied the effects of iron-free lactoferrin (apo LF) and lactoperoxidase system (lactoperoxidase, LP/SCN-/H2O2), separately and together, on the viability of Streptococcus mutans (serotype c) in vitro. The bacteria were incubated in buffered KCl (pH 5.5) with and without the above components which were used at concentrations normally present in human saliva. Both apo LF and LP-system had a bactericidal effect against S. mutans at low pH. Together they showed an additive, but not a synergistic, antibacterial effect against S. mutans. Apo LF enhanced the LP enzyme activity but decreased the yield of the antimicrobial component, hypothiocyanite (HOSCN/OSCN-), when incorporated into the reaction mixtures. This decrease, which was most pronounced at low pH, was due to an LP-independent reaction between apo LF and HOSCN/OSCN-. Our study indicates that the LP-system and apo LF can be combined to combat oral S. mutans.
我们分别及共同研究了无铁乳铁蛋白（脱辅基乳铁蛋白，apo LF）和乳过氧化物酶系统（乳过氧化物酶，LP/SCN⁻/H₂O₂）对变形链球菌（血清型c）体外生存能力的影响。将细菌置于缓冲氯化钾（pH 5.5）中培养，添加或不添加上述成分，其浓度采用人唾液中通常存在的浓度。apo LF和LP系统在低pH值时对变形链球菌均有杀菌作用。二者共同作用时，对变形链球菌显示出相加而非协同的抗菌效果。当apo LF加入反应混合物中时，它增强了LP酶活性，但降低了抗菌成分次硫氰酸盐（HOSCN/OSCN⁻）的产量。这种降低在低pH值时最为明显，是由于apo LF与HOSCN/OSCN⁻之间发生了不依赖于LP的反应。我们的研究表明，LP系统和apo LF可以联合起来对抗口腔中的变形链球菌。
