Płachno Bartosz Jan, Kozieradzka-Kiszkurno Małgorzata, Swiatek Piotr
Department of Plant Cytology and Embryology, Jagiellonian University, 52 Grodzka st., 31-044 Cracow, Poland.
Ann Bot. 2007 Aug;100(2):195-203. doi: 10.1093/aob/mcm109. Epub 2007 Jun 4.
Digestive structures of carnivorous plants produce external digestive enzymes, and play the main role in absorption. In Lentibulariaceae, the ultrastructure of digestive hairs has been examined in some detail in Pinguicula and Utricularia, but the sessile digestive hairs of Genlisea have received very little attention so far. The aim of this study was to fill this gap by expanding their morphological, anatomical and histochemical characterization.
Several imaging techniques were used, including light, confocal and electron microscopy, to reveal the structure and function of the secretory hairs of Genlisea traps. This report demonstrates the application of cryo-SEM for fast imaging of whole, physically fixed plant secretory structures.
The concentration of digestive hairs along vascular bundles in subgenus Genlisea is a primitive feature, indicating its basal position within the genus. Digestive hairs of Genlisea consist of three compartments with different ultrastructure and function. In subgenus Tayloria the terminal hair cells are transfer cells, but not in species of subgenus Genlisea. A digestive pool of viscous fluid occurs in Genlisea traps. In spite of their similar architecture, the digestive-absorptive hairs of Lentibulariaceae feature differences in morphology and ultrastructure.
食虫植物的消化结构会产生外源性消化酶，并在吸收过程中起主要作用。在狸藻科中，捕虫堇属和狸藻属的消化毛超微结构已得到较为详细的研究，但迄今为止，螺旋狸藻属的无柄消化毛很少受到关注。本研究的目的是通过扩展对其形态、解剖和组织化学特征的描述来填补这一空白。
使用了多种成像技术，包括光学显微镜、共聚焦显微镜和电子显微镜，以揭示螺旋狸藻捕虫器分泌毛的结构和功能。本报告展示了冷冻扫描电子显微镜在对完整的、经过物理固定的植物分泌结构进行快速成像方面的应用。
螺旋狸藻亚属中消化毛沿维管束的集中分布是一个原始特征，表明其在该属中的基部位置。螺旋狸藻的消化毛由具有不同超微结构和功能的三个部分组成。在泰勒狸藻亚属中，顶端毛细胞是传递细胞，但在螺旋狸藻亚属的物种中并非如此。螺旋狸藻的捕虫器中有一个粘性消化液池。尽管狸藻科植物的消化吸收毛结构相似，但在形态和超微结构上存在差异。
