Felgines Catherine, Texier Odile, Besson Catherine, Lyan Bernard, Lamaison Jean-Louis, Scalbert Augustin
Université Clermont 1, UFR Pharmacie, Laboratoire de Pharmacognosie, 28 Place Henri Dunant, BP 38, F-63001 Clermont-Ferrand Cedex 1, France.
Br J Nutr. 2007 Dec;98(6):1126-31. doi: 10.1017/S0007114507764772. Epub 2007 Jun 11.
Anthocyanins are natural dietary pigments with a wide array of biological properties that are possibly involved in the prevention of various diseases. These properties depend on their absorption and metabolism in the body. In the present study we first examined the gastric and intestinal absorption of pelargonidin 3-glucoside (Pg 3-glc) using rat in situ models. A high proportion of Pg 3-glc was rapidly absorbed from both the stomach (23 %) and small intestine (24 %). Its metabolism was further studied by feeding rats during 8 d with a diet enriched in freeze-dried strawberries. Only low amounts of total anthocyanins were recovered in 24 h urine (0.163 (SEM 0.013) % of ingested anthocyanins; n 8). Strawberry anthocyanins were analysed in urine by HPLC-electrospray ionisation-tandem MS. Similar proportions of intact glycosides (about 53 %) and glucuronidated metabolites (about 47 %) were found. Pg 3-glc was thus glucuronidated to a larger extent than cyanidin 3-glucoside. These results highlight the influence of the aglycone structure on anthocyanin metabolism.
花青素是具有多种生物学特性的天然膳食色素，可能参与预防多种疾病。这些特性取决于它们在体内的吸收和代谢。在本研究中，我们首先使用大鼠原位模型研究了天竺葵素3 - 葡萄糖苷（Pg 3 - glc）的胃和肠道吸收情况。很大一部分Pg 3 - glc能迅速从胃（23%）和小肠（24%）吸收。通过给大鼠喂食富含冻干草莓的饮食8天，进一步研究了其代谢情况。在24小时尿液中仅回收了少量的总花青素（占摄入花青素的0.163（标准误0.013）%；n = 8）。通过高效液相色谱 - 电喷雾电离 - 串联质谱法分析尿液中的草莓花青素。发现完整糖苷（约53%）和葡萄糖醛酸化代谢物（约47%）的比例相似。因此，Pg 3 - glc的葡萄糖醛酸化程度比矢车菊素3 - 葡萄糖苷更大。这些结果突出了苷元结构对花青素代谢的影响。
