Felgines Catherine, Talavéra Séverine, Texier Odile, Besson Catherine, Fogliano Vincenzo, Lamaison Jean-Louis, la Fauci Luca, Galvano Giacomo, Rémésy Christian, Galvano Fabio
Laboratoire de Pharmacognosie, Faculté de Pharmacie, Clermont-Ferrand, France.
Br J Nutr. 2006 May;95(5):898-904. doi: 10.1079/bjn20061728.
Anthocyanins are natural pigments that could be involved in various health effects. Red oranges are an important dietary source of anthocyanins, including cyanidin 3-glucoside (Cy 3-glc) and an acylated derivative, cyanidin 3-(6''-malonyl)-glucoside (Cy 3-malglc). The aim of this study was to evaluate the absorption and metabolism of red orange anthocyanins in rats fed an anthocyanin-enriched diet for 12 d (approximately 2.8 micromol anthocyanins/d). Furthermore, the absorption of these anthocyanins was studied in both the stomach and intestine using in situ models in rats. Anthocyanin metabolites were identified and quantified by HPLC-electrospray ionization tandem MS and HPLC-diode array detection, respectively. The red orange anthocyanins, Cy 3-glc and Cy 3-malglc, as well as their respective methylated derivatives, were recovered in urine after red orange juice intake. The 24 h urinary excretion of total anthocyanins was low (0.081 (SEM 0.009) % of the ingested amount). However, a high proportion (about 20 %) of red orange anthocyanins was absorbed from the stomach. More Cy 3-malglc than Cy 3-glc was absorbed in the intestine. This study thus indicated that red orange juice anthocyanins were rapidly absorbed from both stomach and small intestine, and then excreted in the urine as intact and methylated forms. Moreover, the absorption and metabolism of acylated anthocyanins and non-acylated anthocyanins were similar.
花青素是一种天然色素，可能具有多种健康功效。血橙是花青素的重要膳食来源，包括矢车菊素3 - 葡萄糖苷（Cy 3 - glc）和一种酰化衍生物矢车菊素3 -（6'' - 丙二酰基）- 葡萄糖苷（Cy 3 - malglc）。本研究的目的是评估在给予富含花青素饮食12天（约2.8微摩尔花青素/天）的大鼠中，血橙花青素的吸收和代谢情况。此外，利用大鼠原位模型研究了这些花青素在胃和肠道中的吸收情况。分别通过高效液相色谱 - 电喷雾电离串联质谱和高效液相色谱 - 二极管阵列检测对花青素代谢产物进行鉴定和定量。摄入血橙汁后，尿液中检测到血橙花青素、Cy 3 - glc和Cy 3 - malglc及其各自的甲基化衍生物。总花青素的24小时尿排泄量较低（占摄入量的0.081（标准误0.009）%）。然而，相当一部分（约20%）的血橙花青素是从胃中吸收的。在肠道中，Cy 3 - malglc的吸收量比Cy 3 - glc多。因此，本研究表明血橙汁中的花青素能迅速从胃和小肠吸收，然后以完整形式和甲基化形式随尿液排出。此外，酰化花青素和非酰化花青素的吸收和代谢情况相似。
