Dauguet Julien, Delzescaux Thierry, Condé Françoise, Mangin Jean-François, Ayache Nicholas, Hantraye Philippe, Frouin Vincent
Service Hospitalier Frédéric Joliot, CEA, Orsay, France.
J Neurosci Methods. 2007 Aug 15;164(1):191-204. doi: 10.1016/j.jneumeth.2007.04.017. Epub 2007 May 1.
The correlation between post-mortem data and in-vivo brain images is of high interest for studying neurodegenerative diseases. This paper describes a protocol that matches a series of stained histological slices of a baboon brain with an anatomical MRI scan of the same subject using an intermediate 3D-consistent volume of "blockface" photographs taken during the sectioning process. Each stained histological section of the baboon brain was first registered to its corresponding blockface photograph using a novel "hemi-rigid" transformation. This piecewise rigid 2D transformation was specifically adapted to the registration of slices which contained both hemispheres. Subsenquently, to correct the global 3D deformations of the brain caused by histological preparation and fixation, a 3D elastic transformation was estimated between the blockface volume and the MRI data. This 3D elastic transformation was then applied to the histological volume previously aligned using the hemi-rigid method to complete the registration of the series of stained histological slices with the MRI data. We assessed the efficacy of our method by evaluating the quality of matching of anatomical features as well as the difference of volume measurements between the MRI and the histological images. Two complete baboon brains (with the exception of cerebellum) were successfully processed using our protocol.
死后数据与活体脑图像之间的相关性对于研究神经退行性疾病具有重要意义。本文描述了一种方案，该方案通过在切片过程中拍摄的一系列“块面”照片的中间3D一致体积，将狒狒大脑的一系列染色组织学切片与同一受试者的解剖MRI扫描进行匹配。狒狒大脑的每个染色组织学切片首先使用一种新颖的“半刚性”变换配准到其相应的块面照片上。这种分段刚性二维变换专门适用于包含两个半球的切片的配准。随后，为了校正由组织学制备和固定引起的大脑整体3D变形，在块面体积和MRI数据之间估计了一个3D弹性变换。然后将这个3D弹性变换应用于先前使用半刚性方法对齐的组织学体积，以完成一系列染色组织学切片与MRI数据的配准。我们通过评估解剖特征匹配的质量以及MRI和组织学图像之间体积测量的差异来评估我们方法的有效性。使用我们的方案成功处理了两个完整的狒狒大脑（小脑除外）。
