Schurr A, Payne R S
Department of Anesthesiology and Perioperative Medicine, University of Louisville, School of Medicine, Louisville, KY 40202, USA.
Neuroscience. 2007 Jul 13;147(3):613-9. doi: 10.1016/j.neuroscience.2007.05.002. Epub 2007 Jun 8.
For over 60 years, a distinction has been made between aerobic and anaerobic glycolysis based on their respective end products: pyruvate of the former, lactate of the latter. Recently we hypothesized that, in the brain, both aerobic and anaerobic glycolysis terminate with the formation of lactate from pyruvate by the enzyme lactate dehydrogenase (LDH). If this hypothesis is correct, lactate must be the mitochondrial substrate for oxidative energy metabolism via its oxidation to pyruvate, plausibly by a mitochondrial LDH. Here we employed electrophysiology of the rat hippocampal slice preparation to test and monitor the effects of malonate and oxamate, two different LDH inhibitors, and glutamate, a neuronal activator, in experiments, the results of which support the hypothesis that lactate, at least in this in vitro setting, is indeed the principal end product of neuronal aerobic glycolysis.
60多年来，有氧糖酵解和无氧糖酵解一直根据它们各自的终产物来区分：前者的终产物是丙酮酸，后者的终产物是乳酸。最近我们推测，在大脑中，有氧糖酵解和无氧糖酵解最终都会通过乳酸脱氢酶（LDH）将丙酮酸转化为乳酸。如果这个假设是正确的，那么乳酸必然是通过其氧化为丙酮酸来作为氧化能量代谢的线粒体底物，很可能是由线粒体LDH催化。在这里，我们利用大鼠海马脑片制备的电生理学方法，在实验中测试并监测了两种不同的LDH抑制剂丙二酸和草氨酸盐以及神经元激活剂谷氨酸的作用，实验结果支持了这样一个假设，即至少在这种体外环境中，乳酸确实是神经元有氧糖酵解的主要终产物。
