含锌氟化羟基磷灰石薄膜的体外生物活性及成骨样细胞测试

In vitro bioactivity and osteoblast-like cell test of zinc containing fluoridated hydroxyapatite films.

作者信息

Miao Shundong, Weng Wenjian, Cheng Kui, Du Piyi, Shen Ge, Han Gaorong, Huang Xin, Yan Weiqi, Zhang Sam

机构信息

Department of Materials Science & Engineering, Zhejiang University, Hangzhou 310009, China.

出版信息

J Mater Sci Mater Med. 2007 Oct;18(10):2101-5. doi: 10.1007/s10856-007-3131-5. Epub 2007 Jun 12.

DOI:10.1007/s10856-007-3131-5

PMID:17562136

Abstract

Zinc containing fluoridated hydroxyapatite (ZnFHA) films on Ti6Al4V substrates was prepared using sol-gel dip-coating method. The release of zinc ions from ZnFHA film was controlled mainly by the zinc content in the film. The release behavior showed an initial rapid increase release followed by a tapering-off and directed to a constant value at longer time. After soaking in SBF for 8 days, a layer was deposited and completely covered the original surface of the ZnFHA film, indicating good in vitro "bioactivity." The osteoblast-like MG63 cells were seeded on the ZnFHA films; FHA film and Ti6Al4V substrate were used as control. The cell culture result showed that cell adhesion and proliferation on ZnFHA films were significantly increased compared with the controls. The results in this work suggest that ZnFHA films on Ti6Al4V substrates can function as an implant with good bioactivity and cytocompatibility.

摘要

采用溶胶 - 凝胶浸涂法在Ti6Al4V基体上制备了含锌的氟化羟基磷灰石（ZnFHA）薄膜。ZnFHA薄膜中锌离子的释放主要受薄膜中锌含量的控制。释放行为表现为最初快速增加，随后逐渐减少，并在较长时间后趋于恒定值。在模拟体液（SBF）中浸泡8天后，沉积了一层并完全覆盖了ZnFHA薄膜的原始表面，表明具有良好的体外“生物活性”。将成骨样MG63细胞接种在ZnFHA薄膜上；以FHA薄膜和Ti6Al4V基体作为对照。细胞培养结果表明，与对照相比，ZnFHA薄膜上的细胞黏附和增殖明显增加。这项工作的结果表明，Ti6Al4V基体上的ZnFHA薄膜可作为具有良好生物活性和细胞相容性的植入物发挥作用。