Lee Seung Yoon, Park Ki-Ho, Choi Jung-Woo, Kwon Jung-Kyun, Lee Doo Rak, Shin Mi Sun, Lee Jee Sung, You Chung Eui, Park Mi Youn
Department of Dermatology, National Medical Center, 18-79, Euljiro 6-ga, Jung-ku, Seoul, Republic of Korea.
J Photochem Photobiol B. 2007 Jul 27;88(1):51-67. doi: 10.1016/j.jphotobiol.2007.04.008. Epub 2007 May 1.
Light-emitting diodes (LEDs) are considered to be effective in skin rejuvenation. We investigated the clinical efficacy of LED phototherapy for skin rejuvenation through the comparison with three different treatment parameters and a control, and also examined the LED-induced histological, ultrastructural, and biochemical changes. Seventy-six patients with facial wrinkles were treated with quasimonochromatic LED devices on the right half of their faces. All subjects were randomly divided into four groups treated with either 830nm alone, 633nm alone, a combination of 830 and 633nm, or a sham treatment light, twice a week for four weeks. Serial photography, profilometry, and objective measurements of the skin elasticity and melanin were performed during the treatment period with a three-month follow-up period. The subject's and investigator's assessments were double-blinded. Skin specimens were evaluated for the histologic and ultrastructural changes, alteration in the status of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs), and the changes in the mRNA levels of IL-1ss, TNF-alpha, ICAM-1, IL-6 and connexin 43 (Cx43), by utilizing specific stains, TEM, immunohistochemistry, and real-time RT-PCR, respectively. In the results, objectively measured data showed significant reductions of wrinkles (maximum: 36%) and increases of skin elasticity (maximum: 19%) compared to baseline on the treated face in the three treatment groups. Histologically, a marked increase in the amount of collagen and elastic fibers in all treatment groups was observed. Ultrastructural examination demonstrated highly activated fibroblasts, surrounded by abundant elastic and collagen fibers. Immunohistochemistry showed an increase of TIMP-1 and 2. RT-PCR results showed the mRNA levels of IL-1ss, TNF-alpha, ICAM-1, and Cx43 increased after LED phototherapy whereas that of IL-6 decreased. This therapy was well-tolerated by all patients with no adverse effects. We concluded that 830 and 633nm LED phototherapy is an effective approach for skin rejuvenation.
发光二极管（LED）被认为在皮肤年轻化方面具有有效性。我们通过与三种不同治疗参数及一个对照组进行比较，研究了LED光疗用于皮肤年轻化的临床疗效，并且还检测了LED诱导的组织学、超微结构及生化变化。76名面部有皱纹的患者其右脸接受了准单色LED设备治疗。所有受试者被随机分为四组，分别接受单独的830nm光、单独的633nm光、830nm与633nm光联合治疗，或假治疗光，每周两次，共四周。在治疗期间进行连续摄影、轮廓测定以及皮肤弹性和黑色素的客观测量，并进行为期三个月的随访。受试者和研究者的评估均采用双盲法。利用特异性染色、透射电子显微镜（TEM）、免疫组织化学和实时逆转录聚合酶链反应（RT-PCR），分别对皮肤标本的组织学和超微结构变化、基质金属蛋白酶（MMPs）及其组织抑制剂（TIMPs）状态的改变以及白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、细胞间黏附分子-1（ICAM-1）、白细胞介素-6（IL-6）和连接蛋白43（Cx43）的mRNA水平变化进行评估。结果显示，与基线相比，三个治疗组治疗面部的客观测量数据显示皱纹显著减少（最多减少36%），皮肤弹性增加（最多增加19%）。组织学上，所有治疗组的胶原蛋白和弹性纤维数量均显著增加。超微结构检查显示成纤维细胞高度活化，周围有丰富的弹性和胶原纤维。免疫组织化学显示TIMP-1和TIMP-2增加。RT-PCR结果显示，LED光疗后IL-1β、TNF-α、ICAM-1和Cx43的mRNA水平升高，而IL-6的mRNA水平降低。所有患者对该治疗耐受性良好，无不良反应。我们得出结论，830nm和633nm LED光疗是一种有效的皮肤年轻化方法。
