Isenhath S N, Fukano Y, Usui M L, Underwood R A, Irvin C A, Marshall A J, Hauch K D, Ratner B D, Fleckman P, Olerud J E
Department of Medicine/Dermatology, University of Washington, Seattle, Washington.
Department of BioEngineering, University of Washington, Seattle, Washington.
J Biomed Mater Res A. 2007 Dec 15;83(4):915-922. doi: 10.1002/jbm.a.31391.
Percutaneous medical devices are integral in the management and treatment of disease. The space created between the skin and the device becomes a haven for bacterial invasion and biofilm formation and results in infection. We hypothesize that sealing this space via integration of the skin into the device will create a barrier against bacterial invasion. The purpose of this study was to develop an animal model in which the interaction between skin and biomaterials can be evaluated. Porous poly(2-hydroxyethyl methacrylate) [poly(HEMA)] rods were implanted for 7 days in the dorsal skin of C57 BL/6 mice. The porous poly(HEMA) rods were surface-modified with carbonyldiimidazole (CDI) or CDI plus laminin 5; unmodified rods served as control. Implant sites were sealed with 2-octyl cyanoacrylate; corn pads and adhesive dressings were tested for stabilization of implants. All rods remained intact for the duration of the study. There was histological evidence of both epidermal and dermal integration into all poly(HEMA) rods regardless of treatment. This in vivo model permits examination of the implant/skin interface and will be useful for future studies designed to facilitate skin cell attachment where percutaneous devices penetrate the skin.
经皮医疗器械在疾病的管理和治疗中不可或缺。皮肤与器械之间形成的空间成为细菌入侵和生物膜形成的温床，进而导致感染。我们推测，通过将皮肤与器械整合来封闭该空间可形成一道抵御细菌入侵的屏障。本研究的目的是建立一种可评估皮肤与生物材料之间相互作用的动物模型。将多孔聚（甲基丙烯酸羟乙酯）[聚（HEMA）]棒植入C57 BL/6小鼠的背部皮肤7天。多孔聚（HEMA）棒用羰基二咪唑（CDI）或CDI加层粘连蛋白5进行表面改性；未改性的棒用作对照。植入部位用2-氰基丙烯酸辛酯封闭；测试了玉米垫和粘性敷料对植入物的固定效果。在研究期间所有棒均保持完整。无论何种处理，均有组织学证据表明表皮和真皮均整合到所有聚（HEMA）棒中。这种体内模型允许检查植入物/皮肤界面，将有助于未来旨在促进经皮器械穿透皮肤处皮肤细胞附着的研究。
