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一种商用麻疹病毒免疫球蛋白M酶免疫测定法的评估

Evaluation of a commercial measles virus immunoglobulin M enzyme immunoassay.

作者信息

Mayo D R, Brennan T, Cormier D P, Hadler J, Lamb P

机构信息

Biosciences Division, Connecticut State Department of Health Services, Hartford 06144.

出版信息

J Clin Microbiol. 1991 Dec;29(12):2865-7. doi: 10.1128/jcm.29.12.2865-2867.1991.

Abstract

Paired serum samples from 93 patients suspected of having measles were assayed for measles virus-specific immunoglobulin M (IgM) antibodies by an enzyme immunoassay (EIA), and the results were compared with results from a complement fixation assay and an EIA for measles virus IgG. By using significant serologic rises as the standard for comparison, the IgM EIA assay had a sensitivity of 85.7%, a specificity of 81.3%, a positive predictive value of 95.7%, and a negative predictive value of 54.2%. This assay can be expected to perform well in outbreak situations.

摘要

采用酶免疫分析法(EIA)对93例疑似麻疹患者的配对血清样本进行麻疹病毒特异性免疫球蛋白M(IgM)抗体检测,并将结果与补体结合试验及麻疹病毒IgG的EIA结果进行比较。以显著的血清学升高作为比较标准,IgM EIA检测的灵敏度为85.7%,特异性为81.3%,阳性预测值为95.7%,阴性预测值为54.2%。预计该检测方法在疫情暴发时表现良好。

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