Godsey J H, Matteo M R, Shen D, Tolman G, Gohlke J R
J Clin Microbiol. 1981 Mar;13(3):483-90. doi: 10.1128/jcm.13.3.483-490.1981.
A total of 539 clinical isolates belonging to 10 species of the Enterobacteriaceae family were identified by enzyme activity profiles within 30 min of test inoculation. Each isolate was grown at 37 degrees C for 18 h on Mueller-Hinton agar and suspended to an optical density of 200 Klett units on 0.85% saline. Enzyme activity profiles were obtained by inoculating 18 fluorogenic substrates with the standardized bacterial suspension and monitoring initial rates of hydrolysis over the first 30 min of analysis. Individual enzyme activity profiles were entered into a coded data bank, and identifications were based on the Bayesian theory of probabilities. At a confidence level of 95%, five species were identified with a greater than 90% efficiency, three species were identified between 83 and 88% efficiency, and two species demonstrated a 72 and 75% efficiency of identification. The enzyme activity profile method of bacterial identification is rapid, easily automated, and reproducible.
在接种测试后30分钟内，通过酶活性谱鉴定出539株属于肠杆菌科10个种的临床分离株。将每个分离株在穆勒-欣顿琼脂上于37℃培养18小时，并悬浮于0.85%盐水中，使其光密度达到200 Klett单位。通过用标准化细菌悬液接种18种荧光底物并监测分析的前30分钟内的初始水解速率来获得酶活性谱。将各个酶活性谱输入编码数据库，并基于贝叶斯概率理论进行鉴定。在95%的置信水平下，5个种的鉴定效率大于90%，3个种的鉴定效率在83%至88%之间，2个种的鉴定效率分别为72%和75%。细菌鉴定的酶活性谱方法快速、易于自动化且可重复。
