Gorelov V N, Gubina E A, Grekova N A, Skavronskaia A G
Zh Mikrobiol Epidemiol Immunobiol. 1991 Sep(9):2-4.
The possibility of preparing B. abortus vaccine strain 19-BA with multiresistance to antibiotics was shown. The strain was obtained by the spontaneous induction of resistance to rifampicin with the subsequent transformation of nonconjugative hybrid plasmid pOVI which, in addition to rifampicin resistance, governed the resistance of brucellae to tetracycline, doxycycline, ampicillin, and streptomycin. Experiments on guinea pigs demonstrated the immunization with both multiresistant vaccine strain GSA1 and B. abortus initial vaccine strain 19-BA.
已证明制备对多种抗生素具有多重抗性的流产布鲁氏菌疫苗株19-BA是可能的。该菌株是通过对利福平的自发诱导抗性获得的，随后非结合型杂交质粒pOVI发生转化，该质粒除了赋予对利福平的抗性外，还控制布鲁氏菌对四环素、强力霉素、氨苄青霉素和链霉素的抗性。对豚鼠的实验表明，用多重抗性疫苗株GSA1和流产布鲁氏菌初始疫苗株19-BA进行免疫均有效。
