Gómez-Ambrosi Javier, Catalán Victoria, Ramírez Beatriz, Rodríguez Amaia, Colina Inmaculada, Silva Camilo, Rotellar Fernando, Mugueta Carmen, Gil María J, Cienfuegos Javier A, Salvador Javier, Frühbeck Gema
Metabolic Research Laboratory, Clínica Universitaria de Navarra, University of Navarra, 31008 Pamplona, Spain.
J Clin Endocrinol Metab. 2007 Sep;92(9):3719-27. doi: 10.1210/jc.2007-0349. Epub 2007 Jun 26.
Obesity acts as a cardiovascular risk factor by mechanisms that are not fully understood. Osteopontin (OPN) is a proinflammatory mediator involved in tissue remodeling that plays a role in atherosclerosis and diabetes.
The aim of the present study was to compare the circulating concentrations of OPN and its mRNA expression in omental adipose tissue of lean, overweight, and obese individuals and to analyze the effect of weight loss.
Plasma concentrations of OPN were measured in 77 volunteers. OPN mRNA expression in omental adipose tissue obtained from 12 women was quantified by real-time PCR. In addition, the concentrations of OPN in 12 obese men were measured before and after weight loss following a dietetic program.
The study was conducted at a University Hospital.
Obese and overweight patients exhibited significantly increased circulating OPN concentrations as compared with lean subjects (obese 72.6 +/- 28.5, overweight 68.2 +/- 20.8, lean 42.7 +/- 27.9 ng/ml; P < 0.001). A significant positive correlation was found between OPN levels and body fat (r = 0.45; P < 0.0001). Obese individuals showed significantly increased (P < 0.05) mRNA expression of OPN in omental adipose tissue as compared with lean volunteers, which was further increased in obese diabetic patients. Diet-induced weight loss significantly decreased OPN concentrations from 64.7 +/- 22.1 to 36.6 +/- 20.1 ng/ml (P = 0.006).
These findings represent the first observation that plasma OPN and mRNA expression of OPN in omental adipose tissue are increased in overweight/obese patients with the latter being further elevated in obesity-associated diabetes. Moreover, weight loss reduces OPN concentrations, which may contribute to the beneficial effects accompanying weight reduction. Measurement of OPN might be useful for evaluating the outcomes of various clinical interventions for obesity-related cardiovascular diseases.
肥胖通过尚未完全明确的机制成为心血管危险因素。骨桥蛋白（OPN）是一种参与组织重塑的促炎介质，在动脉粥样硬化和糖尿病中发挥作用。
本研究旨在比较瘦人、超重者和肥胖者网膜脂肪组织中OPN的循环浓度及其mRNA表达，并分析体重减轻的影响。
检测了77名志愿者血浆中OPN的浓度。通过实时PCR定量分析了12名女性网膜脂肪组织中OPN mRNA的表达。此外，还对12名肥胖男性在遵循饮食计划减肥前后的OPN浓度进行了检测。
该研究在一家大学医院进行。
与瘦人相比，肥胖和超重患者的循环OPN浓度显著升高（肥胖者72.6±28.5，超重者68.2±20.8，瘦人42.7±27.9 ng/ml；P<0.001）。OPN水平与体脂之间存在显著正相关（r = 0.45；P<0.0001）。与瘦志愿者相比，肥胖个体网膜脂肪组织中OPN的mRNA表达显著增加（P<0.05），在肥胖糖尿病患者中进一步升高。饮食诱导的体重减轻使OPN浓度从64.7±22.1显著降至36.6±20.1 ng/ml（P = 0.006）。
这些发现首次表明，超重/肥胖患者血浆OPN和网膜脂肪组织中OPN的mRNA表达增加，在肥胖相关糖尿病患者中后者进一步升高。此外，体重减轻可降低OPN浓度，这可能有助于减轻体重带来的有益影响。检测OPN可能有助于评估肥胖相关心血管疾病各种临床干预的效果。
