Zhang L, Hu M, Jones A, Allsopp B A, Beveridge I, Schindler A R, Gasser R B
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia.
Mol Cell Probes. 2007 Oct-Dec;21(5-6):379-85. doi: 10.1016/j.mcp.2007.05.003. Epub 2007 May 21.
In the present study, we have extended earlier taxonomic, biochemical and experimental investigations to characterize two species of Taenia from carnivores in Kenya by use of the sequences of a variable domain (D1) of nuclear ribosomal DNA and the cytochrome c oxidase subunit 1 and NADH dehydrogenase 1 genes of mitochondrial DNA. Emphasis was placed on the characterization of Taenia madoquae from the silver-backed jackal (Canis mesomelas) and Taenia regis from the lion (Panthera leo), given the previous absence of any DNA sequence data for them, and on assessing their genetic relationships with socioeconomically important taeniids. The study showed that T. regis was genetically most closely related to T. hydatigena, and T. madoquae to T. serialis, T. multiceps or T. saginata. The present findings provide a stimulus for future work on the systematic relationships and epidemiology of lesser-known taeniid cestodes in Africa and other continents, employing mitochondrial sequence data sets.
在本研究中，我们扩展了早期的分类学、生物化学和实验研究，通过使用核糖体DNA可变区（D1）序列以及线粒体DNA的细胞色素c氧化酶亚基1和NADH脱氢酶1基因，对肯尼亚食肉动物体内的两种带绦虫进行特征描述。鉴于此前没有关于银背胡狼（Canis mesomelas）体内的马多夸带绦虫（Taenia madoquae）和狮子（Panthera leo）体内的雷吉斯带绦虫（Taenia regis）的任何DNA序列数据，重点对它们进行了特征描述，并评估它们与具有社会经济重要性的带绦虫的遗传关系。研究表明，雷吉斯带绦虫在基因上与泡状带绦虫（Taenia hydatigena）关系最为密切，而马多夸带绦虫与连续带绦虫（Taenia serialis）、多头带绦虫（Taenia multiceps）或牛带绦虫（Taenia saginata）关系密切。本研究结果为利用线粒体序列数据集开展非洲及其他大陆鲜为人知的带绦虫绦虫系统关系和流行病学的未来研究提供了动力。
