Huang Pei-Jane, Huang Yi-Chau, Su Mei-Fang, Yang Tzu-Yi, Huang Jone-Ray, Jiang Cho-Pei
Biomedical Electronics Technology Division, Medical Electronics and Device Technology Center, Industrial Technology Research Institute, Hsinchu, Taiwan, R.O.C.
Photomed Laser Surg. 2007 Jun;25(3):183-90. doi: 10.1089/pho.2007.2062.
The purpose of this study was to evaluate the influence of Cu-GHK aids for the LED-PI on fibroblast proliferation and collagen production in vitro.
Light-emitting diode photoirradiation (LED-PI) and copper-glycyl-L-histidyl-L-lysine complex (Cu-GHK) treatment may be useful in accelerating the rate of wound healing. Red LED (625-635 nm) was used as a light source for LED-PI. In the process of wound healing, Cu-GHK was shown to be an activator of remodeling. LED-PI would maintain fibroblast activity and viability, and there would be a positive effect on type I collagen (COL1) and basic fibroblast growth factor (bFGF) production from the combination of LED-PI and Cu-GHK incorporation.
Cell activity/viability, procollagen type I C-peptide (P1CP), and bFGF were evaluated in vitro with human fibroblasts (HS68). The effects of single factors (LED-PI using 0, 1, and 2 J energy doses) or a combination of factors (LED-PI and Cu-GHK) on fibroblast viability (i.e., alamarBlue reduction), collagen production (i.e., P1CP production and COL1 mRNA expression), and bFGF secretion were also evaluated.
Reduction in cell viability was significantly suppressed with LED-PI (1 J) and Cu-GHK-supplied incubation. Cell viability was increased 12.5-fold compared with the non-irradiated group (0 J). Collagen production was also increased significantly with LED-PI and Cu-GHK incorporation (197.6 ng/mL). A dose-response effect was observed for LED-PI combined with Cu-GHK. The combinative effects of LED-PI and Cu-GHK led to an increase not only in bFGF secretion (approximately 230%) but also in P1CP production (approximately 30%) and COL1 mRNA expression (approximately 70%) compared with LED-PI alone.
LED-PI maintained human fibroblast (HS68) viability and increased collagen synthesis when applied by itself. In the combinative stimulation for in vitro collagen production (when LED-PI was followed by Cu-GHK-supplied incubation), stimulated cells showed increased bFGF secretion, P1CP production, and COL1 expression, compared to the LED-PI treatment alone.
本研究旨在评估铜-甘氨酰-L-组氨酰-L-赖氨酸配合物（Cu-GHK）辅助发光二极管光辐照（LED-PI）对体外成纤维细胞增殖和胶原蛋白生成的影响。
发光二极管光辐照（LED-PI）和铜-甘氨酰-L-组氨酰-L-赖氨酸配合物（Cu-GHK）治疗可能有助于加快伤口愈合速度。红色发光二极管（625 - 635纳米）用作LED-PI的光源。在伤口愈合过程中，Cu-GHK被证明是重塑过程的激活剂。LED-PI可维持成纤维细胞的活性和活力，LED-PI与Cu-GHK联合使用对I型胶原蛋白（COL1）和碱性成纤维细胞生长因子（bFGF）的生成有积极影响。
用人成纤维细胞（HS68）在体外评估细胞活性/活力、I型前胶原C肽（P1CP）和bFGF。还评估了单一因素（使用0、1和2焦耳能量剂量的LED-PI）或因素组合（LED-PI和Cu-GHK）对成纤维细胞活力（即alamarBlue还原）、胶原蛋白生成（即P1CP生成和COL1 mRNA表达）和bFGF分泌的影响。
LED-PI（1焦耳）和Cu-GHK孵育显著抑制了细胞活力的降低。与未辐照组（0焦耳）相比，细胞活力提高了12.5倍。LED-PI与Cu-GHK联合使用也显著增加了胶原蛋白生成（197.6纳克/毫升）。观察到LED-PI与Cu-GHK联合使用存在剂量反应效应。与单独使用LED-PI相比，LED-PI与Cu-GHK的联合作用不仅导致bFGF分泌增加（约230%），还导致P1CP生成增加（约30%）和COL1 mRNA表达增加（约70%）。
单独应用时，LED-PI可维持人成纤维细胞（HS68）的活力并增加胶原蛋白合成。在体外胶原蛋白生成的联合刺激中（LED-PI后进行Cu-GHK孵育），与单独的LED-PI处理相比，受刺激的细胞显示出bFGF分泌、P1CP生成和COL1表达增加。
