Kursk State Medical University, Ministry of Health of the Russian Federation, Kursk, Russia.
Bull Exp Biol Med. 2024 Jan;176(3):411-416. doi: 10.1007/s10517-024-06035-w. Epub 2024 Feb 12.
We evaluated the effects of the Gly-His-Lys-D-Ala peptide in a dose 0.5 μg/kg on skin wound regeneration in male Wistar rats (n=80) after initial surgical debridement when administered intracutaneously around the site of injury. Histological (severity of the inflammatory reaction, formation of granulation tissue, and epithelialization terms) and morphometric (number of fibroblastic cells, macrophages, granulocytes, and lymphocytes) studies were performed on autopsy specimens on days 3, 7, 10, and 30 of the experiment. Daily intracutaneous injection of the peptide resulted in an increase in the number of fibroblastic cells and macrophages, as well as in a decrease in the number of granulocytes against the background of active wound contraction on day 30 of the experiment. Thus, Gly-His-Lys-D-Ala alleviated the inflammatory reaction and promoted the regenerative processes.
我们评估了甘氨酰-组氨酰-赖氨酸-D-丙氨酸肽（剂量为 0.5μg/kg）对雄性 Wistar 大鼠（n=80）在初始外科清创后通过皮内注射至损伤部位周围对皮肤伤口再生的影响。在实验的第 3、7、10 和 30 天，对尸检标本进行了组织学（炎症反应严重程度、肉芽组织形成和上皮化术语）和形态计量学（成纤维细胞、巨噬细胞、粒细胞和淋巴细胞的数量）研究。每天皮内注射该肽可在实验第 30 天增加成纤维细胞和巨噬细胞的数量，并减少粒细胞数量，同时伴有活跃的伤口收缩。因此，甘氨酰-组氨酰-赖氨酸-D-丙氨酸肽减轻了炎症反应并促进了再生过程。
