Müller Vanessa, Chávez Juliana H, Reginatto Flávio H, Zucolotto Silvana M, Niero Rivaldo, Navarro Dionezine, Yunes Rosendo A, Schenkel Eloir P, Barardi Célia R M, Zanetti Carlos R, Simões Cláudia M O
Departamento de Ciências Farmacêuticas, CCS, Universidade Federal de Santa Catarina (UFSC), Florianópolis 88040-900, SC, Brasil.
Phytother Res. 2007 Oct;21(10):970-4. doi: 10.1002/ptr.2198.
This paper describes the screening of different South American plant extracts and fractions. Aqueous and organic extracts were prepared and tested for antiherpetic (HSV-1, KOS and 29R strains) and antirabies (PV strain) activities. The evaluation of the potential antiviral activity of these extracts was performed by using an MTT assay for HSV-1, and by a viral cytopathic effect (CPE) inhibitory method for rabies virus (RV). The results were expressed as 50% cytotoxicity (CC(50)) for MTT assay and 50% effective (EC(50)) concentrations for CPE, and with them it was possible to calculate the selectivity indices (SI = CC(50)/EC(50)) of each tested material. From the 18 extracts/fractions tested, six extracts and four fractions showed antiviral action. Ilex paraguariensis, Lafoensia pacari, Passiflora edulis, Rubus imperialis and Slonea guianensis showed values of SI > 7 against HSV-1 KOS and 29-R strains and Alamanda schottii showed a SI of 5.6 against RV, PV strain.
本文描述了对不同南美植物提取物和馏分的筛选。制备了水提取物和有机提取物，并测试了其抗疱疹病毒（单纯疱疹病毒1型，KOS和29R毒株）和抗狂犬病（PV毒株）活性。通过MTT法对单纯疱疹病毒1型进行检测，以及通过病毒致细胞病变效应（CPE）抑制法对狂犬病病毒（RV）进行检测，来评估这些提取物的潜在抗病毒活性。结果以MTT法的50%细胞毒性（CC(50)）和CPE的50%有效（EC(50)）浓度表示，并据此计算每种受试材料的选择性指数（SI = CC(50)/EC(50)）。在测试的18种提取物/馏分中，有6种提取物和4种馏分显示出抗病毒作用。巴拉圭冬青、拉福恩西亚、西番莲、帝王悬钩子和圭亚那斯隆木对单纯疱疹病毒1型KOS和29-R毒株的SI值>7，而肖特黄蝉对狂犬病病毒PV毒株的SI值为5.6。
