Varady K A, Roohk D J, Loe Y C, McEvoy-Hein B K, Hellerstein M K
Department of Nutritional Sciences and Toxicology, University of California at Berkeley, Berkeley, CA 94720, USA.
J Lipid Res. 2007 Oct;48(10):2212-9. doi: 10.1194/jlr.M700223-JLR200. Epub 2007 Jul 2.
Calorie restriction (CR) affects adipocyte function and reduces body weight. However, the effects of alternate-day fasting (ADF) on adipose biology remain unclear. This study examined the effects of ADF and modified ADF regimens on adipocyte size, triglyceride (TG) metabolism, and adiponectin levels in relation to changes in body weight and adipose mass. Twenty-four male C57BL/6J mice were randomized for 4 weeks among 1) ADF-25% (25% CR on fast day, ad libitum on alternate day), 2) ADF-50% (50% CR on fast day), 3) ADF-100% (100% CR on fast day), and 4) control (ad libitum). The body weight of ADF-100% mice was lower than that of the other groups (P < 0.005) after treatment. Adipose tissue weights did not change. Inguinal and epididymal fat cells were 35-50% smaller (P < 0.01) than those of controls in ADF-50% and ADF-100% animals after treatment. Net lipolysis was augmented (P < 0.05) in ADF-100% mice, and the contribution from glyceroneogenesis to alpha-glycerol phosphate increased in ADF-50% and ADF-100% mice, whereas fractional and absolute de novo lipogenesis also increased in ADF-50% and ADF-100% animals, consistent with an alternating feast-fast milieu. Plasma adiponectin levels were not affected. In summary, modified ADF (ADF-50%) and complete ADF (ADF-100%) regimens modulate adipocyte function, despite there being no change in body weight or adipose tissue weight in the former group.
热量限制（CR）会影响脂肪细胞功能并减轻体重。然而，隔日禁食（ADF）对脂肪生物学的影响仍不清楚。本研究探讨了ADF和改良ADF方案对脂肪细胞大小、甘油三酯（TG）代谢以及脂联素水平的影响，并分析了其与体重和脂肪量变化的关系。将24只雄性C57BL/6J小鼠随机分为4组，进行为期4周的实验：1）ADF-25%组（禁食日热量摄入减少25%，非禁食日自由进食），2）ADF-50%组（禁食日热量摄入减少50%），3）ADF-100%组（禁食日完全禁食），4）对照组（自由进食）。治疗后，ADF-100%组小鼠的体重低于其他组（P < 0.005）。脂肪组织重量没有变化。治疗后，ADF-50%组和ADF-100%组小鼠腹股沟和附睾脂肪细胞比对照组小35 - 50%（P < 0.01）。ADF-100%组小鼠的净脂肪分解增加（P < 0.05），ADF-50%组和ADF-100%组小鼠中甘油生成对α-磷酸甘油的贡献增加，而ADF-50%组和ADF-100%组动物的从头脂肪生成分数和绝对量也增加，这与交替的 feast-fast 环境一致。血浆脂联素水平未受影响。总之，改良ADF（ADF-50%）和完全ADF（ADF-100%）方案可调节脂肪细胞功能，尽管前一组的体重和脂肪组织重量没有变化。
