Wang Ting, Zhang Jin-chao, Chen Yao, Huang Feng, Yang Meng-su, Xiao Pei-gen
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China.
Zhongguo Zhong Yao Za Zhi. 2007 Apr;32(8):715-8.
To study the antioxidative and antitumor activities of flavonoids isolated from Epimedium koreanum.
The compounds were separated by column chromatography with silica gel and Sephadex LH-20, and identified by spectral a- nalysis (ESI-MS, 1H-NMR and 13C-NMR) respectively. DPPH radical scavenging assay and MTT assay were used to observe the antioxidative and antitumor abilities.
Six compounds were isolated from the the ethyl acetate extract of the aerial part. Their structures were identified as icariin (I), luteolin (II), baohuoside II (III), hyperoside (IV), epimedokoreanin B (V) and baohuoside I (VI). The results indicated that at concentrations of 3. 125-200 micromol x L(-1), compound I, III and VI had no ability to scavenge the DPPH radical, but the scavenging ability of compounds II, IV and V were stronger than that of Vit C in dose-dependant manner. Compounds I, II, V and VI could inhibit the proliferation of MCF-7 and HepG2 in dose-dependant manner, but compounds III and IV had no effect on the proliferation.
The antitumor activity of E. koreanum may be partially related to the antioxidantive activity of flavonoids.
研究朝鲜淫羊藿中黄酮类化合物的抗氧化和抗肿瘤活性。
采用硅胶柱色谱和葡聚糖凝胶LH - 20柱色谱分离化合物，分别通过光谱分析（电喷雾电离质谱、氢核磁共振和碳核磁共振）进行鉴定。采用二苯基苦味酰基自由基清除试验和MTT试验观察其抗氧化和抗肿瘤能力。
从地上部分的乙酸乙酯提取物中分离得到6种化合物。其结构鉴定为淫羊藿苷（I）、木犀草素（II）、宝藿苷II（III）、金丝桃苷（IV）、朝鲜淫羊藿素B（V）和宝藿苷I（VI）。结果表明，在3.125 - 200微摩尔·升⁻¹浓度范围内，化合物I、III和VI没有清除二苯基苦味酰基自由基的能力，但化合物II、IV和V的清除能力呈剂量依赖性，且强于维生素C。化合物I、II、V和VI能呈剂量依赖性抑制MCF - 7和HepG2细胞的增殖，但化合物III和IV对细胞增殖无影响。
朝鲜淫羊藿的抗肿瘤活性可能部分与其黄酮类化合物的抗氧化活性有关。
