朝鲜淫羊藿总黄酮及黄酮醇苷对原代成骨细胞增殖与分化的影响
Effects of total flavonoids and flavonol glycosides from Epimedium koreanum Nakai on the proliferation and differentiation of primary osteoblasts.
作者信息
Zhang D-W, Cheng Y, Wang N-L, Zhang J-C, Yang M-S, Yao X-S
机构信息
Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
出版信息
Phytomedicine. 2008 Jan;15(1-2):55-61. doi: 10.1016/j.phymed.2007.04.002. Epub 2007 May 4.
In a bioassay-guided drug screening for anti-osteoporosis activity, eight flavonol glycosides were isolated from Epimedium koreanum Nakai, which is traditionally widely used in China for the treatment of impotence and osteoporosis. The effects of total flavonoids and flavonol glycosides on the proliferation and differentiation of rat calvarial osteoblast-like cells were evaluated by the MTT method and measuring the activity of alkaline phosphatase (ALP activity). Total flavonoids (1.2 x10(-2) to 6.0 x10(-7) mg/ml) and flavonol glycosides (2.0 x10(-5) to 1.0 x10(-9) mol/l) exhibited a strong inhibition on the proliferation of primary osteoblasts at most concentrations. However, the total flavonoids and icariin significantly promoted the differentiation of primary osteoblasts. The results suggested that flavonoids from E. koreanum Nakai may improve the development of osteoblasts by promoting the ALP activity; and icariin might be one of the active constituents facilitating the differentiation of osteoblasts.
在一项针对抗骨质疏松活性的生物测定指导下的药物筛选中，从朝鲜淫羊藿中分离出了8种黄酮醇苷，朝鲜淫羊藿在中国传统上被广泛用于治疗阳痿和骨质疏松症。采用MTT法并通过测量碱性磷酸酶活性（ALP活性），评估了总黄酮和黄酮醇苷对大鼠颅骨成骨样细胞增殖和分化的影响。总黄酮（1.2×10⁻²至6.0×10⁻⁷mg/ml）和黄酮醇苷（2.0×10⁻⁵至1.0×10⁻⁹mol/l）在大多数浓度下对原代成骨细胞的增殖均表现出强烈抑制作用。然而，总黄酮和淫羊藿苷显著促进了原代成骨细胞的分化。结果表明，朝鲜淫羊藿中的黄酮类化合物可能通过促进ALP活性来改善成骨细胞的发育；淫羊藿苷可能是促进成骨细胞分化的活性成分之一。
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