Department of Natural Product Chemistry, Key Laboratory of Chemical Biology, Ministry of Education, School of Pharmaceutical Sciences, Shandong University, Jinan, P. R. China.
Nat Prod Res. 2020 May;34(9):1256-1263. doi: 10.1080/14786419.2018.1560283. Epub 2019 Jan 19.
Phytochemical studies on the leaves of Nakai have resulted in the discovery of two new flavonol glycosides, koreanoside F () and koreanoside G (), along with six known flavonoids. Their structures were elucidated on the basis of HRESIMS, UV, IR, 1 D NMR and 2 D NMR data. Absolute configurations of and was further determined by C-NMR spectra with gate decoupling (GD). All of the compounds were evaluated for cytotoxic activities by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. The results indicated that compounds , , , and inhibited the proliferation of A549 and NCI-292 cells with IC values of 5.7-23.5 μM. Real-time monitoring in three kinds of lung cancer cells and a kind of human bronchial epithelial cells treated with compound was also assessed.
对 Nakai 叶的植物化学研究导致发现了两种新的类黄酮糖苷，朝鲜糖苷 F（）和朝鲜糖苷 G（），以及六种已知的类黄酮。基于高分辨率电喷雾质谱（HRESIMS）、紫外（UV）、红外（IR）、一维 NMR 和二维 NMR 数据阐明了它们的结构。和的绝对构型通过带有门控去耦（GD）的碳核磁共振谱（C-NMR）进一步确定。所有化合物均通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-四唑溴化物（MTT）测定法评估了对 A549 和 NCI-292 细胞的细胞毒性活性。结果表明，化合物、、、和抑制 A549 和 NCI-292 细胞的增殖，IC 值为 5.7-23.5 μM。还评估了用化合物处理三种肺癌细胞和一种人支气管上皮细胞的实时监测。
