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Evaluation of the An-Ident system and an indole spot test for the rapid differentiation of porcine treponemes.

作者信息

Bélanger M, Jacques M

机构信息

Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.

出版信息

J Clin Microbiol. 1991 Aug;29(8):1727-9. doi: 10.1128/jcm.29.8.1727-1729.1991.

DOI:10.1128/jcm.29.8.1727-1729.1991
PMID:1761697
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC270192/
Abstract

The An-Ident strip system (Analytab Products Inc., St-Laurent, Quebec, Canada) was evaluated for its ability to differentiate Treponema hyodysenteriae from Treponema innocens. Of the 20 tests included on this strip, 15 yielded identical results for the two species. Among the other five assays, none could be considered as a positive discriminator for the two species. However, when an indole spot test with 1% p-dimethylaminocinnamaldehyde was used in parallel, all reference strains and 97% of the isolates of T. hyodysenteriae were positive, whereas all isolates of T. innocens were negative. Our results indicate that An-Indent is of little value for the differentiation of the two species. Our results also suggest that a rapid and simple differentiation between T. hyodysenteriae and T. innocens can be achieved by using the hemolysis and ring phenomenon tests in conjunction with an indole spot test.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b83/270192/5fd43905c689/jcm00044-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b83/270192/5fd43905c689/jcm00044-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b83/270192/5fd43905c689/jcm00044-0194-a.jpg

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本文引用的文献

1
Differentiation of Treponema hyodysenteriae from T innocens by enteropathogenicity testing in the CF1 mouse.
Vet Rec. 1980 Dec 6;107(23):527-9.
2
Identification of Treponema hyodysenteriae by a rapid slide agglutination test.通过快速玻片凝集试验鉴定猪痢疾密螺旋体。
Vet Rec. 1981 Feb 28;108(9):187-9. doi: 10.1136/vr.108.9.187.
3
[Comparative study of the biochemical characteristics of hemolytic treponemes isolated from pigs].[从猪分离出的溶血性密螺旋体生化特性的比较研究]
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J Clin Microbiol. 1993 Mar;31(3):711-6. doi: 10.1128/jcm.31.3.711-716.1993.
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Rapid detection of Serpulina hyodysenteriae in diagnostic specimens by PCR.通过聚合酶链反应(PCR)快速检测诊断标本中的猪痢疾蛇形螺旋体。
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Porcine colonic spirochetosis: a retrospective study of eleven cases.猪结肠螺旋体病:11例回顾性研究
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6
Serotyping of Canadian isolates of Treponema hyodysenteriae and description of two new serotypes.猪痢疾密螺旋体加拿大分离株的血清分型及两种新血清型的描述。
J Clin Microbiol. 1991 Dec;29(12):2794-7. doi: 10.1128/jcm.29.12.2794-2797.1991.
7
Molecular characterization of Serpulina (Treponema) hyodysenteriae isolates representing serotypes 8 and 9.代表血清型8和9的猪痢疾蛇形螺旋体(密螺旋体)分离株的分子特征
J Clin Microbiol. 1992 Nov;30(11):2941-7. doi: 10.1128/jcm.30.11.2941-2947.1992.
4
Comparison of stained smears and culturing for identification of Treponema hyodysenteriae.用于鉴定猪痢疾密螺旋体的染色涂片与培养方法的比较。
J Clin Microbiol. 1983 Oct;18(4):950-5. doi: 10.1128/jcm.18.4.950-955.1983.
5
The production of dysentery in swine by feeding cultures containing a spirochaete.通过喂食含有螺旋体的培养物使猪患痢疾。
Br Vet J. 1971 Nov;127(11):58-61. doi: 10.1016/s0007-1935(17)37282-2.
6
Evaluation of media and reagents for indole-spot tests in anaerobic bacteriology.厌氧细菌学中吲哚斑点试验的培养基和试剂评估
Am J Clin Pathol. 1972 Sep;58(3):335-8. doi: 10.1093/ajcp/58.3.335.
7
Inoculation of pigs with Treponema hyodysenteriae (new species) and reproduction f the disease.用猪痢疾密螺旋体(新种)接种猪并使该病重现。
Vet Med Small Anim Clin. 1972 Jan;67(1):61-4.
8
Isolation and propagation of spirochetes from the colon of swine dysentery affected pigs.从患猪痢疾的猪的结肠中分离和培养螺旋体。
Can J Comp Med. 1972 Jan;36(1):74-6.
9
New serotypes of Treponema hyodysenteriae.猪痢疾密螺旋体新血清型。
J Clin Microbiol. 1985 Aug;22(2):161-4. doi: 10.1128/jcm.22.2.161-164.1985.
10
API ZYM and API An-Ident reactions of fastidious oral gram-negative species.苛求性口腔革兰氏阴性菌的API ZYM和API An-Ident反应
J Clin Microbiol. 1985 Sep;22(3):333-5. doi: 10.1128/jcm.22.3.333-335.1985.