Doebbe Anja, Rupprecht Jens, Beckmann Julia, Mussgnug Jan H, Hallmann Armin, Hankamer Ben, Kruse Olaf
Department of Biology, Algae Biotech Group, University Bielefeld, Bielefeld, Germany.
J Biotechnol. 2007 Aug 1;131(1):27-33. doi: 10.1016/j.jbiotec.2007.05.017. Epub 2007 May 24.
Phototrophic organisms use photosynthesis to convert solar energy into chemical energy. In nature, the chemical energy is stored in a diverse range of biopolymers. These sunlight-derived, energy-rich biopolymers can be converted into environmentally clean and CO(2) neutral fuels. A select group of photosynthetic microorganisms have developed the ability to extract and divert protons and electrons derived from water to chloroplast hydrogenase(s) to produce molecular H(2) fuel. Here, we describe the development and characterization of C. reinhardtii strains, derived from the high H(2) production mutant Stm6, into which the HUP1 (hexose uptake protein) hexose symporter from Chlorella kessleri was introduced. The isolated cell lines can use externally supplied glucose for heterotrophic growth in the dark. More importantly, external glucose supply (1mM) was shown to increase the H(2) production capacity in strain Stm6Glc4 to approximately 150% of that of the high-H(2) producing strain, Stm6. This establishes the foundations for a new fuel production process in which H(2)O and glucose can simultaneously be used for H(2) production. It also opens new perspectives on future strategies for improving bio-H(2) production efficiency under natural day/night regimes and for using sugar waste material for energy production in green algae as photosynthetic catalysts.
光合生物利用光合作用将太阳能转化为化学能。在自然界中，化学能储存在多种生物聚合物中。这些源自阳光、富含能量的生物聚合物可转化为环境清洁且碳中和的燃料。一组特定的光合微生物已具备从水中提取质子和电子并将其转移至叶绿体氢化酶以产生分子氢燃料的能力。在此，我们描述了源自高氢产量突变体Stm6的莱茵衣藻菌株的开发与特性，其中引入了来自小球藻的HUP1（己糖摄取蛋白）己糖同向转运体。分离出的细胞系可利用外部供应的葡萄糖在黑暗中进行异养生长。更重要的是，外部葡萄糖供应（1 mM）显示可使菌株Stm6Glc4的产氢能力提高至高产氢菌株Stm6的约150%。这为一种新的燃料生产工艺奠定了基础，在该工艺中，水和葡萄糖可同时用于产氢。它还为未来在自然昼夜条件下提高生物产氢效率以及利用糖废料在绿藻中作为光合催化剂进行能源生产的策略开辟了新的前景。
