Instituto Superior de Agronomia/Centro de Botânica Aplicada à Agricultura, Calçada da Tapada, 1349-017 Lisboa, Portugal.
Trends Biotechnol. 2011 Dec;29(12):595-600. doi: 10.1016/j.tibtech.2011.06.008. Epub 2011 Jul 25.
Molecular hydrogen (H(2)) obtained from biological sources provides an alternative to bulk chemical processes that is moving towards large-scale, economical generation of clean fuel for automotive engines. This opinion article examines recent improvements in H(2) production by wild and mutant strains of Chlamydomonas reinhardtii - the green microalga currently considered the best eukaryotic H(2) producer. Here, we review various aspects of genetic and metabolic engineering of C. reinhardtii, as well as of process engineering. Additionally, we lay out possible scenarios that would lead to more efficient research approaches in the near future, as part of a consistent strategy for sustainable biohydrogen supply.
从生物来源获得的分子氢（H(2)）为大规模、经济地生产用于汽车发动机的清洁燃料提供了一种替代批量化学工艺的方法。本文观点文章考察了近期通过野生和突变株莱茵衣藻（目前被认为是最好的真核 H(2)生产者的绿藻）生产 H(2)的改进。在这里，我们回顾了莱茵衣藻的遗传和代谢工程以及工艺工程的各个方面。此外，我们还提出了一些可能的方案，这些方案将作为可持续生物制氢供应的一致策略的一部分，为未来更有效的研究方法提供依据。
