Rainey Fred A, Ferreira Margarida, Nobre M Fernanda, Ray Keren, Bagaley Danielle, Earl Ashlee M, Battista John R, Gómez-Silva Benito, McKay Christopher P, da Costa Milton S
Department of Biological Sciences, 202 Life Sciences Building, Louisiana State University, Baton Rouge, LA 70803, USA.
Departamento de Bioquímica and Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
Int J Syst Evol Microbiol. 2007 Jul;57(Pt 7):1408-1412. doi: 10.1099/ijs.0.64956-0.
Three ionizing-radiation-resistant bacterial strains (designated KR-196, KR-198 and KR-200(T)) were isolated from a sample of arid soil collected from a coastal desert in Chile. The soil sample was irradiated before serial dilution plating was performed using one-tenth-strength plate count agar. Phylogenetic analysis of the 16S rRNA gene sequences showed these organisms to represent a novel species of the genus Deinococcus, having sequence similarities of 87.3-90.8 % with respect to recognized Deinococcus species. Strains KR-196, KR-198 and KR-200(T) were aerobic and showed optimum growth at 30 degrees C and pH 6.5-8.0. The major respiratory menaquinone was MK-8. The predominant fatty acids in these strains were 16 : 1 omega 7c, 16 : 0, 15 : 1 omega 6c, 17 : 0 and 18 : 0. The DNA G+C content of strain KR-200(T) was 63.9 mol%. Strains KR-196, KR-198 and KR-200(T) were found to be resistant to >10 kGy gamma radiation. On the basis of the phylogenetic, chemotaxonomic and phenotypic data, strain KR-200(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus peraridilitoris sp. nov. is proposed. The type strain is KR-200(T) (=LMG 22246(T)=CIP 109416(T)).
从智利沿海沙漠采集的干旱土壤样本中分离出三株抗电离辐射的细菌菌株（分别命名为KR-196、KR-198和KR-200(T)）。在使用十分之一强度平板计数琼脂进行系列稀释平板培养之前，对土壤样本进行了辐照。16S rRNA基因序列的系统发育分析表明，这些微生物代表了嗜皮菌属的一个新物种，与已确认的嗜皮菌属物种的序列相似性为87.3 - 90.8%。菌株KR-196、KR-198和KR-200(T)为需氧菌，在30℃和pH 6.5 - 8.0条件下生长最佳。主要呼吸醌为MK-8。这些菌株中的主要脂肪酸为16 : 1 ω7c、16 : 0、15 : 1 ω6c、17 : 0和18 : 0。菌株KR-200(T)的DNA G+C含量为63.9 mol%。发现菌株KR-196、KR-198和KR-200(T)对>10 kGy的γ辐射具有抗性。基于系统发育、化学分类和表型数据，菌株KR-200(T)代表了嗜皮菌属的一个新物种，为此提出新名称嗜皮菌荒漠新种（Deinococcus peraridilitoris sp. nov.）。模式菌株为KR-200(T)（=LMG 22246(T)=CIP 109416(T)）。
