Azeem Muhammad, Siddique Muhammad Hussnain, Imran Muhammad, Zubair Muhammad, Mumtaz Rabia, Younas Madiha, Abdel-Maksoud Mostafa A, El-Tayeb Mohamed A, Rizwan Muhammad, Yong Jean Wan Hong
Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Faisalabad, 38000, Pakistan.
Department of Environmental Sciences, COMSATS University Islamabad, Vehari-Campus, Vehari, 61100, Pakistan.
Heliyon. 2024 Jul 4;10(14):e34073. doi: 10.1016/j.heliyon.2024.e34073. eCollection 2024 Jul 30.
Cancer and diabetes represent significant challenges in the field of biomedicine, with major and global impacts on public health. , commonly called 'gum arabic tree,' is recognized for its unique biomedical properties. The current study aimed to investigate the pharmacological potential of -based zinc-oxide nanoparticles (ZnO-NPs) in comparison to the ethanol and methanol-based extracts against cancer, diabetes, and oxidative stress. Green synthesis of ZnO-NPs was performed using barks of . Different techniques for the characterization of ZnO-NPs, including UV-Visible spectroscopy, Scanning Electron Microscopy, Fourier Transmission Infrared (FT-IR) spectroscopy, and X-ray Diffraction (XRD), were utilized. The morphological analysis of ZnO-NPs revealed that the fine NPs have mean particle sizes of 15 ± 1.5 nm. For the solvent based-extraction, leaves and barks were utilized and dissolved into ethanol and methanol for further processing. The MTT assay revealed that the optimum concentration of ZnO-NPs to inhibit the proliferation of liver cancer cell line HepG2 was 100 μg/mL where 67.0 % inhibition was observed; and both ethanol- and methanol-based extracts showed optimum inhibition at 100 μg/mL. The DPPH assay further demonstrated that 250 μg/mL of ZnO-NPs and 1000 μg/mL of both ethanol- and methanol-based extracts, as the optimum concentration for antioxidant activity (with 73.1 %, 68.9 % and 68.2 % inhibition respectively). The α-Glucosidase inhibition assay revealed that 250 μg/mL of ZnO-NPs and 10 μg/mL of both ethanol- and methanol-based extracts as the optimum concentration for antidiabetic activity (with 95 %, 93.7 % and 93.4 % inhibition respectively). The study provided interesting insights into the efficacy and reliability of ZnO-NPs for potential pharmacological application. Further research should be focused on examining specific pathways and the safety of ZnO-NPs in comparison to solvent-based extracts.
癌症和糖尿病是生物医学领域的重大挑战，对公众健康产生重大的全球性影响。 ，通常被称为“阿拉伯胶树”，因其独特的生物医学特性而闻名。本研究旨在研究基于 的氧化锌纳米颗粒（ZnO-NPs）与乙醇和甲醇提取物相比在抗癌、抗糖尿病和氧化应激方面的药理潜力。使用 的树皮进行ZnO-NPs的绿色合成。采用了不同的技术对ZnO-NPs进行表征，包括紫外可见光谱、扫描电子显微镜、傅里叶透射红外（FT-IR）光谱和X射线衍射（XRD）。ZnO-NPs的形态分析表明，细纳米颗粒的平均粒径为15±1.5nm。对于溶剂萃取，使用树叶和树皮并将其溶解在乙醇和甲醇中进行进一步处理。MTT试验表明，抑制肝癌细胞系HepG2增殖的ZnO-NPs最佳浓度为100μg/mL，观察到67.0%的抑制率；乙醇和甲醇提取物在100μg/mL时均显示出最佳抑制效果。DPPH试验进一步表明，250μg/mL的ZnO-NPs以及1000μg/mL的乙醇和甲醇提取物是抗氧化活性的最佳浓度（分别具有73.1%、68.9%和68.2%的抑制率）。α-葡萄糖苷酶抑制试验表明，250μg/mL的ZnO-NPs以及10μg/mL的乙醇和甲醇提取物是抗糖尿病活性的最佳浓度（分别具有95%、93.7%和93.4%的抑制率）。该研究为ZnO-NPs在潜在药理应用中的功效和可靠性提供了有趣的见解。进一步的研究应集中在研究特定途径以及与溶剂提取物相比ZnO-NPs的安全性上。
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