Riddell Michaela A, Moss William J, Hauer Debra, Monze Mwaka, Griffin Diane E
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Baltimore, MD 21205, USA.
J Clin Virol. 2007 Aug;39(4):312-7. doi: 10.1016/j.jcv.2007.05.006. Epub 2007 Jul 10.
Measles virus (MV) RNA was detected 1 month after hospitalization with measles in more than half of Zambian children but the duration of detectable RNA was not determined.
To characterize the time course of MV clearance and identify factors associated with presence of viral RNA at late times after clinical recovery from infection.
Blood, urine and nasopharyngeal specimens from 49 Zambian children with laboratory-confirmed measles were collected a median of 100 days (range 65-118) after rash onset. Samples were assayed for MV nucleocapsid and hemagglutinin RNA by reverse transcriptase-polymerase chain reaction. Amplified products were sequenced. Selected immunologic studies were performed.
MV RNA was detected in at least one specimen from 18 children (37%). Eighteen percent of 44 blood mononuclear cell, 23% of 30 nasopharyngeal and 50% of 6 urine specimens were positive. Detection was not associated with HIV-1 infection, % CD4(+) T lymphocytes, plasma interleukin-10 levels or persistent MV-specific IgM. The MV genotype was D2 and sequences of late specimens were the same as specimens collected during acute illness.
Presence of viral RNA at multiple sites more than 3 months after acute disease suggests that clearance of MV-infected cells occurs over many months.
超过半数的赞比亚麻疹患儿在住院1个月后仍可检测到麻疹病毒（MV）RNA，但未确定可检测到RNA的持续时间。
描述MV清除的时间进程，并确定感染临床恢复后晚期病毒RNA存在的相关因素。
收集49例实验室确诊为麻疹的赞比亚儿童在出疹后中位时间100天（范围65 - 118天）的血液、尿液和鼻咽标本。通过逆转录聚合酶链反应检测样本中的MV核衣壳和血凝素RNA。对扩增产物进行测序，并进行了选定的免疫学研究。
18名儿童（37%）的至少一份标本中检测到MV RNA。44份血液单核细胞标本中有18%、30份鼻咽标本中有23%、6份尿液标本中有50%呈阳性。检测结果与HIV-1感染、CD4(+) T淋巴细胞百分比、血浆白细胞介素-10水平或持续性MV特异性IgM无关。MV基因型为D2，晚期标本的序列与急性疾病期间采集的标本相同。
急性疾病3个月后多个部位存在病毒RNA表明，MV感染细胞的清除需要数月时间。
