Rezende T M B, Vieira L Q, Cardoso F P, Oliveira R R, de Oliveira Mendes S T, Jorge M L R, Ribeiro Sobrinho A P
Departamento de Dentística Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Int Endod J. 2007 Aug;40(8):603-11. doi: 10.1111/j.1365-2591.2007.01255.x.
To assess the influence of co-culture with mineral trioxide aggregate (MTA) on phagocytosis and the production of reactive oxygen intermediates (ROI) and nitrogen (NO) species and the arginase activity by M1 and M2 peritoneal macrophages.
Cellular viability, adherence and phagocytosis of Saccharomyces boulardii were assayed in the presence of MTA. Macrophages were stimulated with zymosan for ROI assays and with Fusobacterium nucleatum and Peptostreptococcus anaerobius and IFN-gamma for NO production and arginase activity, when in contact with capillaries containing MTA. Data were analysed by T, anova, Kruskall-Wallis and Mann-Whitney tests.
M2 macrophages displayed greater cellular viability in polypropylene tubes, greater ability to ingest yeast and smaller production of ROI and higher arginase activity when compared with M1 macrophages. Both macrophages, M1 and M2, presented similar cell adherence and NO production. The addition of bacterial preparations to macrophages interfered with NO and arginase productions. MTA did not interfere with any of the parameters measured.
Phagocytosis and the ability of the two macrophage subtypes to eliminate microbes were not affected by MTA.
评估与三氧化矿物凝聚体（MTA）共培养对M1和M2腹腔巨噬细胞吞噬作用、活性氧中间体（ROI）和氮（NO）物质生成以及精氨酸酶活性的影响。
在存在MTA的情况下，测定布拉酵母菌的细胞活力、黏附及吞噬作用。当与含有MTA的毛细管接触时，用酵母聚糖刺激巨噬细胞进行ROI检测，用具核梭杆菌、厌氧消化链球菌和干扰素-γ刺激巨噬细胞检测NO生成及精氨酸酶活性。数据采用T检验、方差分析、Kruskal-Wallis检验和Mann-Whitney检验进行分析。
与M1巨噬细胞相比，M2巨噬细胞在聚丙烯管中表现出更高的细胞活力、更强的酵母摄取能力、更低的ROI生成量及更高的精氨酸酶活性。M1和M2巨噬细胞的细胞黏附及NO生成情况相似。向巨噬细胞中添加细菌制剂会干扰NO生成及精氨酸酶活性。MTA对所测的任何参数均无干扰。
MTA不影响两种巨噬细胞亚型的吞噬作用及清除微生物的能力。
