Kang A S, Jones T M, Burton D R
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11120-3. doi: 10.1073/pnas.88.24.11120.
A number of experiments on the shuffling of heavy and light chains from antibodies of defined specificity for the transition-state analogue hapten nitrophenyl phosphonamidate are described. The experiments report on the promiscuity of heavy and light chains in binding antigen and the feasibility of antibody redesign by this shuffling process. The concepts of incestuous and extraclonal promiscuous association are described. Shuffling opens the possibility of generating panels of antibodies with related specificity but of distinct idiotypic composition that may have significance in the use of human monoclonal antibodies in therapy.
本文描述了一系列关于对过渡态类似物半抗原硝基苯基膦酰胺具有特定特异性的抗体的重链和轻链改组的实验。这些实验报告了重链和轻链在结合抗原方面的混杂性以及通过这种改组过程进行抗体重新设计的可行性。文中还描述了近亲混杂关联和克隆外混杂关联的概念。改组为生成具有相关特异性但独特的独特型组成的抗体库开辟了可能性，这在人类单克隆抗体用于治疗方面可能具有重要意义。
