Caton A J, Koprowski H
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Proc Natl Acad Sci U S A. 1990 Aug;87(16):6450-4. doi: 10.1073/pnas.87.16.6450.
Antibodies specific for the influenza virus hemagglutinin have been isolated from a combinatorial expression library generated using mRNA obtained from an immunized donor mouse. Sequence analysis indicates that the antibody heavy chain variable regions were derived from members of an expanded hemagglutinin-specific B-cell clone, in conjunction with one of two light chain variable regions. Moreover, the most frequently identified heavy chain variable/light chain variable combination is extremely similar to a heavy chain variable/light chain variable combination that has previously been identified among hemagglutinin-specific hybridoma antibodies. The results, therefore, demonstrate that these antibodies bear a close relationship to the immune status of the donor mouse and suggest that simple adaptations of this procedure might allow evaluation of the immune responses of species, such as man, in which conventional hybridoma techniques have to date proven ineffective.
已从使用免疫供体小鼠的mRNA构建的组合表达文库中分离出针对流感病毒血凝素的特异性抗体。序列分析表明，抗体重链可变区源自一个扩增的血凝素特异性B细胞克隆的成员，并与两个轻链可变区之一结合。此外，最常鉴定到的重链可变区/轻链可变区组合与先前在血凝素特异性杂交瘤抗体中鉴定到的重链可变区/轻链可变区组合极为相似。因此，这些结果表明这些抗体与供体小鼠的免疫状态密切相关，并表明对该方法进行简单调整可能有助于评估诸如人类等物种的免疫反应，而传统杂交瘤技术迄今在这些物种中已证明无效。
