Tan T Y, Ng L S Y, Kwang L L
Division of Laboratory Medicine, Changi General Hospital, Singapore.
J Clin Pathol. 2008 Mar;61(3):343-6. doi: 10.1136/jcp.2007.050757. Epub 2007 Jul 14.
To evaluate the accuracy of direct disc susceptibility testing performed from positive BACTEC blood culture vials, using a predetermined dilution protocol.
Direct susceptibility testing was performed from 432 positive blood culture vials, generating 3829 antibiotic-organism results. Results were compared with those obtained by standard disc susceptibility testing according to Clinical Laboratory Standards Institute (CLSI) methods.
When results were compared with the reference method, no very major errors were detected. One (0.03%) major error and 89 (2.3%) minor errors were found. Error rates by organism group ranged from 1.3% for Pseudomonas aeruginosa to 8.2% for beta-haemolytic streptococci.
Direct susceptibility testing provided accurate susceptibility results for most organism-antibiotic combinations, with the exception of the beta-haemolytic streptococci.
使用预先确定的稀释方案，评估从阳性BACTEC血培养瓶进行直接药敏试验的准确性。
从432个阳性血培养瓶中进行直接药敏试验，得出3829个抗生素-微生物结果。根据临床实验室标准协会（CLSI）方法，将结果与通过标准纸片扩散法药敏试验获得的结果进行比较。
将结果与参考方法进行比较时，未检测到非常重大的错误。发现1例（0.03%）重大错误和89例（2.3%）轻微错误。按微生物类别划分的错误率范围为，铜绿假单胞菌为1.3%，β溶血性链球菌为8.2%。
除β溶血性链球菌外，直接药敏试验为大多数微生物-抗生素组合提供了准确的药敏结果。
