Dang Hai-zhen, Wei Pin-kang, Zhang Xiao-feng, Liu Zhan-wen
Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Zhong Xi Yi Jie He Xue Bao. 2007 Jul;5(4):437-41. doi: 10.3736/jcim20070415.
To observe the effects of warming spleen and stomach for dispersing phlegm recipe (Wenzhong Xiaotan Recipe, WZXTR), a compound traditional Chinese herbal medicine, on expression of interleukin-8 (IL-8) in S(180) tumor tissue in mice, and to explore the effectiveness and its mechanism.
Fifty mice were randomly divided into five groups: untreated group, cold water-treated group, cold water and WZXTR-treated group, cold water and dispersing phlegm and eliminating stagnation recipe (Xiaotan Sanjie Recipe, XTSJR, another compound traditional Chinese herbal medicine) treated group, cold water and tegafur-treated group. Mice in the latter 4 groups were fed 0-4 degrees centigrade cold distilled water 10 ml/(kg x d) one week before tumor inoculation for 3 weeks. Mice in the 5 groups were inoculated neoplastic cells which were diluted 2 x 10(7)/ml with normal saline in the right armpit at the second week. Forty-eight hours later, the mice were given drugs intragastrically (WZXTR, XTSJR and tegafur, respectively). The mice were continually intervened by cold water in the morning and given drugs in the afternoon. Mice in the cold water-treated group were given normal saline, and nothing was given in untreated group. Morphology appearance and changes of rectal temperature were observed, and the tumor weight and volume were measured and inhibitory rate was calculated 22 days after tumor inoculation. IL-8 was detected by avidin-biotin system-enzyme-labeled immunosorbent assay (ABC-ELISA) and real-time reverse polymerase chain reaction (real-time PCR).
The content of IL-8 in tumor tissue of the untreated group was significantly higher than that of the other groups (P<0.01), and that of the cold water-treated group was higher than that of the cold water and WZXTR-treated group (P<0.01) and the cold water and XTSJR-treated group (P<0.05), respectively. The gray scale value of cDNA amplification strap was descent more evidently in both the cold water and WZXTR-treated group and the cold water and XTSJR-treated group as compared with that of the cold water-treated group and the untreated group (P<0.05).
WZXTR can restrain tumor growth, and the mechanism may be that it can degrade the expression of IL-8 in S180 tumor.
观察中药复方温中止痰方对小鼠S（180）肿瘤组织白细胞介素-8（IL-8）表达的影响，探讨其疗效及作用机制。
将50只小鼠随机分为5组：空白组、冷水组、冷水加温中止痰方组、冷水加化痰散结方组、冷水加替加氟组。后4组小鼠于接种肿瘤前1周开始给予0～4℃冷蒸馏水10 ml/（kg·d），连续3周。5组小鼠均于第2周在右腋窝接种用生理盐水稀释至2×10（7）/ml的瘤细胞。48小时后，分别给予温中止痰方、化痰散结方及替加氟灌胃。冷水组小鼠上午继续给予冷水，下午给予生理盐水，空白组不给予任何处理。观察小鼠形态外观及直肠温度变化，接种肿瘤22天后测量肿瘤重量和体积，计算抑瘤率。采用抗生物素蛋白-生物素系统-酶联免疫吸附测定法（ABC-ELISA）及实时逆转录聚合酶链反应（real-time PCR）检测IL-8。
空白组肿瘤组织IL-8含量显著高于其他各组（P<0.01），冷水组高于冷水加温中止痰方组（P<0.01）及冷水加化痰散结方组（P<0.05）。与冷水组和空白组比较，冷水加温中止痰方组和冷水加化痰散结方组cDNA扩增条带灰度值下降更明显（P<0.05）。
温中止痰方可抑制肿瘤生长，其机制可能是下调S180肿瘤组织中IL-8的表达。
